Report on examination of the catalogues on the freezers in the MP archive, room

Transkript

1 Annex Report on examination of the catalogues on the freezers in the MP archive, room April 2012 the Panel inspected the freezers in the MP archive, including the then newly found - 80 freezer. The Secretariat had in advance found some slide trays in one of the (old) freezers which may have relevance for the Panel s investigation based on the labelling of these trays/slides (labelling with keys which have been identified as maybe-relevant for the papers selected by the Panel). However, a basic precondition for being maybe-relevant in relation to the papers selected by the Panel is that the material is slides stained by conventional immunocytochemistry, because the data in the selected papers are derived from slides thus stained. Slides stained by conventional immunocytochemistry are normally not stored on freeze. The Panel therefore assumed that the maybe-relevant slides in the freezers are either unstained or stained by immunoflourescence, neither of which are relevant to the papers selected by the Panel , the Secretariat received from SUND the files containing the records on the contents of the freezers in room in the MP archive. In order to ensure a complete examination of the freezers for maybe-relevant materials and to ensure that the Panel conducts analyses on all found maybe-relevant material, the Secretariat examined these files and identified, on basis of the labelling of the material, all material which may be relevant background material to scientific papers selected by the Panel, in agreement with the Panel Chair. The records on the thus mayberelevant material in the freezers are specified in tables 1 and 2 further below. Table 1 shows the maybe-relevant slides recorded to be placed in the Kummefryser (i.e. the minus 80 degrees freezer found in March-April 2012 by SUND). Table 2 shows the maybe-relevant slides recorded to be placed in the Fryser Vestfrost, earlier called the Left freezer. These slides in the Fryser Vestfrost were identified by the Secretariat already as maybe-associated with some of the papers selected by the Panel see table 3. The files recording the content in the Kummefryser also contain records on further tissue labelled with some of the keys (DD, D, Ø and BKL) that has been identified as maybe-relevant for the papers selected by the Panel. These records are seen in table 4. However, in the investigation, as regards primary material, the Panel has focused on examining slides (stained by conventional immunocytochemistry). The tissue recorded in table 4 comprises human biopsies and other material contained in plastic tubes and plastic containers, and is thus not relevant for further analysis by the Panel.

2 Subsequent to the examination of the catalogue, the Panel asked SUND to inform whether any of the maybe-relevant material in the freezers is stained by conventional immunocytochemistry. According to SUND s response of 16 May 2012 the slides in question in the -80 freezer are all unstained slides, and the slides in question in The left freezer (fryser Vestfrost) are all stained by immunoflourescence. The Panel thus concludes that none of the slides in the freezers can be identified as being of relevance in relation to the Panel s investigation. Table 1. Records on primary material which may be of interest for the Panel s examination, in the Freezer Kummefryser (provided the slides are stained by conventional immunocytochemistry) Type Beskrivelse/Titel Indhold Løst i kummefryseren [Placed freely in the freezer] : Sort kasse [A33] Sort kasse [A211] Markeret med: Ruths KOLprojekt, JAN Markeret med: Henriette Pilegaard, Feb 2005 Slides Slides

3 Table 2. Records on slides which may be of interest for the Panel s examination, in The left freezer (fryser Vestfrost) 1) (provided the slides are stained by conventional immunocytochemistry) Type Beskrivelse/Titel Indhold Hylde 1 (fra oven): Slidebakker 8/04/2008. Human, Diffust store B-celle Lymfom, Slides Hidalgo + CD 34 A Slidebakker Ingen Dato. Human, Diffust Store celle. Hidalgo + Slides CDC 47. Second.?? Bilofeduolopi Slidebakker 3/04/2008. Fluorescent, Hidalgo + 8-oxy??. Human Slides diffust store B-celle Lymfom Slidebakker 11/03/2008. Diffust store celle. Hidalgo +???. Slides Secou. Datou F0205 Slidebakker 4/04/2008. Human, diffust store B-celle lymfom, Slides Hidalgo + p53 Slidebakker 25/03/2010. MT Cubrun, Human tissue,??? Slides storcellet B-celle lymfom Hylde 2 (fra oven): Slidebakker 28/09/2007. Human tissue, Hodyins, whole part??? Slides Slidebakker 18/03/2009. Caspese 3 + MT I?II red, Hodginks Slides Slidebakker 24/03/2009. MDA + MT I+II red. Hodgins Slides Slidebakke Human Hodgins, Megalin + MT I+II Slides Slidebakke Human Hodgins, Cybilin + Megalin Slides Slidebakke Human Hodgins, Cybilin MT I + II Slides 1) The Vestfrost freezer also contains some slides labelled Rikke of diffuse large B cell Lymphoma. However these are not of relevance for the papers selected by the Panel, as Rikke is assumed to be student/co-author Rikke Jensen, none of whose papers the Panel has selected for further examination. Table 3. Slides which may be relevant to papers selected, identified in The left freezer (fryser Vestfrost) by the Secretariat RUM THE LEFT FREEZER Type Beskrivelse/Titel Papers Left freezer, 2 nd and 3 rd shelf Contains stained sections of Hodgkin s lymphoma and Diffuse Large B cell lymphoma A90 A207

4 Table 4. Other tissue labelled with relevant keys in the Freezer Kummefryser (found by SUND March-April 2012) This tissue is other tissue than stained slides and is thus not relevant for Panel s analysis Type Beskrivelse/Titel Indhold Tårn Bagest, Nummer 1 fra venstre Plastikæske, nummer 1 (fra oven) Papæske, nummer 2 (fra oven) Tårn Bagest, Nummer 2 fra venstre Biopsier fra 2 personer. Tilhørende brev til Milena fra Bente, der forklarer prøverne. (ingen dato). Prøverne er navngivet IVL16. Der er 2 poser med 10 små(2-3 ml) plastikrør i hver. BKL. Milena. Muskelbiopsier i tissue Tavie. Muskel/fedt Biopsiprojekt i plastikrør markeret på formen: (bogstav), (tal), (tal), (bogstav). Følgende prøver findes i æsken: Y1 1-H til Y1 6-H (6 stk ialt) X1 1-H til X1 6-H (6 stk ialt) V1 1-H til V1 6-H (6 stk ialt) T1 1-H til T1 6-H (6 stk ialt) S1 1-H til S1 6-H (6 stk ialt) R1 1-H til R1 6-H (6 stk ialt) P1 1-H til P1 6-H (6 stk ialt) N1 1-H til N1 6-H (6 stk ialt) Papæske, nummer 6 (fra oven) Tårn Bagest, Nummer 3 fra venstre Papæske, nummer 6 (fra oven) Mærket: KOL Histo Muskel, Kol + Raske (ingen dato) Papæske markeret: Milena T600 (ingen dato) Indeholder 40 små(1-2 ml) plastikrør. Se Bilag 2 - RUM , Kummefryser På et af prøveglassene er der en dato 22/ Plastikbeholdere (ca 15 ml) markeret henholdsvis: - 602, 17/99-I - 605, 17/99-II - 610, 18/99-DI - 611, 18/99-DD - 614, 19/99-ø - 615, 19/99-I - 620, 20/99-I - 621, 20/99-DD - 632, 20/99-II

5 Papæske, nummer 7 (fra oven) Markeret: Milena T600 (ingen dato) 9 Plastikbeholdere (ca 15 ml) markeret henholdsvis: - 601, 17/99-ø - 603, 17/99-DI - 606, 17/99-DDI - 607, 18/99-ø - 608, 18/99-D - 609, 18/99-I - 612, 18/99-DDI - 617, 19/99-DDI - 619,20/99-ø Tårn Bagest, Nummer 4 fra venstre Papæske, nummer 5 (fra oven) Papæske, nummer 6 (fra oven) Markeret: Nye BKI 3. VIL-6, 21/5, CF M7641, Histo PS. (Histo) A-H,???? 2001 Markeret: Nye BKI 3. IVIL-6, Histo CF M7641,??? 2001 Indeholder 68 små (2-3 ml) Plastikrør med følgende markeringer: - IVIl6, A1/2 Histo - IVIl6, A1/10 Histo (9 stk) - IVIl6, B1/1 Histo - IVIl6, B1/4 Histo (4 stk) - IVIl6, B1/6 Histo - IVIl6, B1/10 Histo (5 stk) - IVIl6, C1 Histo - IVIl6, C4 Histo (4 stk) - IVIl6, C6 Histo (1 stk) - IVIl6, C8 Histo - IVIl6, C10 Histo (3 stk) - IVIl6, D1 Histo - IVIl6, D10 Histo (10 stk) - IVIl6, E1 Histo - IVIl6, E4 Histo (4 stk) - IVIl6, E6 Histo - IVIl6, E10 Histo (5 stk) - IVIl6, F1 Histo - IVIl6, F7 Histo (7 stk) - IVIl6, F9 Histo - IVIl6, F10 Histo (2 stk) - IVIl6, G01 Histo - IVIl6, G02 Histo (2 stk) - IVIl6, G04 H - IVIl6, G10 H (7 stk) - IVIl6, H01 H - IVIl6, H05 H Indeholder 19 små (2-3 ml) Plastikrør med følgende markeringer:

6 - IVIl6, J1 Histo - IVIl6, J10 Histo (10 stk) - IVIl6, K1 Histo - IVIl6, K9 Histo (9 stk) Lille tårn, markeret med Melina Papæske, nummer 3 (fra oven) Papæske, nummer 4 (fra oven) Papæske, nummer 5 (fra oven) [9 plastic containers (appr. 20 ml) Løst i kummefryseren [Placed freely in the freezer] : *ingen mærkning, navn eller dato* *ingen mærkning, navn eller dato* *ingen mærkning, navn eller dato* Indeholder 8 plastikbeholdere(ca. 20 ml) med følgende mærkning: - 238, 39/97-I - 375, 52/98 ø - 376, 52/98-D - 379, 53/98 ø - 381, 53/98 I - 384, 54/98-DD - 385, 55/98-I - 386, 55/98 DD Indeholder 9 plastikbeholdere (ca. 20 ml) med følgende markering: - 242, 44/97-I - 243, 45/97-ø - 272, 1/98-ø - 280, 2/98-II - 377, 52/98 I - 378, 52/98 DD - 380, 53/98 D - 382, 52/98 DI - 383, 54/98 ø Indeholder 9 plastikbeholdere (ca. 20 ml) med følgende markering: - 43, 13/97-ø /97-ø - 184, 31/97-ø - 190, 30/97-ø - 191, 30/97-D - 192, 30/97-I - 193, 30/97-DI - 241, 43/97-I - 244, 45/97-D

7 Sort kasse [although RH probably is Rigshospitalet, the labelling is not sufficiently precise to associate these slides with any particular exp] Markeret med: Fiber type. Remi, AFD 7641, RH (ingen dato) Slides