Biotest Aps er en privat, forskningsbaseret virksomhed inden for mikrobiologi, bioraffinering og fermentering.

Transkript

1 Biotest Aps Biotest Aps er en privat, forskningsbaseret virksomhed inden for mikrobiologi, bioraffinering og fermentering. Biotest s speciale er udviklingsarbejde i laboratorie- og pilotskala samt rådgivning vedrørende patentering og produktudvikling inden for bioraffinering. Biotest har mange års erfaring med proces og produktudvikling samt opstart af nye virksomheder inden for bioteknologisk udnyttelse af restprodukter fra landbrugsindustrien samt inden for fermentering og mikrobiologisk kontrol med produktionsprocesser. Biotest udfører for tiden rådgivning vedrørende etablering af en fermenteringsfabrik baseret på biomasse og er partner i bioraffinaderiprojektet: BIOREF støttet af Det strategiske forskningsråd. Pauli Kiel, Biotest Aps 1

2 Bioraffinering og afgrødevalg Det grønne bioraffinaderi og Agroferm BIOREF: Development of a biorefinery concept for integrated production of biomedicals, biochemicals, feed and fuels from selected plant materials (BIOREF) ( ) Lucerne som råvare til bioraffinaderiet Pauli Kiel, Biotest Aps 2

3 Grønne bioraffinaderier Produktion af energi, foder og højværdiprodukter Pauli Kiel, Biotest Aps 3

4 The green biorefinery Pauli Kiel, Biotest Aps 4

5 Biomass for fermentation Pauli Kiel, Biotest Aps 5

6 Biotest Aps Patent: Method for treating organic waste materials 1999 Fig. 9 GREEN PELLETS DRYING LACTIC ACID FER- MENTATION OR HEATING + ACID PRESSING (COLD OR HOT) GRINDING AND SEPARATION LACTIC ACID FERMENTATION OR HEATING + ACID CENTRIFUGATION CENTRIFUGATION LEAF PROTEIN DRYING GREEN PLANT PRESSED JUICE BROWN JUICE JUICE POTATO FIBRES DEPROTEINISED POTATO JUICE DRYING STARCH POTATO PROTEIN EVAPORATION EVAPORATION CONCEN- TRATED BROWN JUICE CONCEN- TRATED POTATO JUICE MOLASSES, SUGAR OR GLUCOSE SYRUP FERMENTATION: AMINO ACID AND/OR LACTIC ACID OR ACETIC ACID EVAPORATION CALCIUM MAGNESIUM ACETAT SYRUP LYSIN FEED CONCEN- TRATE LYSIN- LACTATE FEED CON- CENTRATE AMMONIUM LACTATE FEED CON- CENTRATE Pauli Kiel, Biotest Aps 6

7 Production of concentrated brown juice Green biomass Brown juice 0-90 m3/h (Av. 40 m3/h 4% DM) ph measuring ph > 5.5 L. salivarius ph < 4.5 ph < 5.5 Green Crop Drying Plant Cooling Tower CSTR 100 m3 Supernatant Fodder pellets Buffer for concentrated brown juice 1,000 m3 Sediment Sedimentation tank 500 m3 Sediment buffer tank 10,000 m3 Storage/buffer tank for acidified brown juice 25,000 m3 Evaporator Storage for concentrated acidified brown juice 6,000 m3 Transport of m3/år (25 % DM) Lysine factory Pauli Kiel, Biotest Aps 7

8 Growth media The growth media contains all necessary components for growth of cells. Either synthetic or complex media are used. Complex media: Corn steep liquor (CSL) Yeast extract Peptone (protein hydrolysate) Brown juice Manure Pauli Kiel, Biotest Aps 8

9 Lysine fermentation Brown juice Ammonia Sugar Amino acids F E R M E N T O R Centrifuge E V A P O R A T I O N Lysine feed concentrate Protein 25 % Pauli Kiel, Biotest Aps 9

10 Inoculation of big fermentor, I Pauli Kiel, Biotest Aps 10

11 Inoculation of big fermentor ( 50 m3) Pauli Kiel, Biotest Aps 11

12 AgroFerm fermentation plant in Esbjerg Pauli Kiel, Biotest Aps 12

13 Development of a biorefinery concept for integrated production of biomedicals, biochemicals, feed and fuels from selected plant materials (BIOREF) ( ) Pauli Kiel, Biotest Aps 13

14 Project Manager: Birgitte K. Ahring Project Coordinator: Peter S. Lübeck Project management team: Birgitte K. Ahring, Peter S. Lübeck, Lene Lange, Michael Givskov, Pauli Kiel, Marie J. Mikkelsen WP 1: Birgitte K. Ahring (AAU, WSU) Biomass materials and Characterization WP 2: Pauli Kiel (Biotest) Feed additives and protein extraction WP 3: Troels Hilstrøm (BioGasol) Pretreatment WP 4: Lene Lange (AAU) Enzyme discovery and expression WP 5: Marie J. Mikkelsen (BioGasol) New biofuel producing bacteria WP 6: Peter S. Lübeck (AAU) New enzymes and Biochemical producing fungi WP 7: Michael Givskov (KU) Identification of biomedical products WP 8: Jens Iversen (BioGasol) Pilot testing Pauli Kiel, Biotest Aps 14

15 Pauli Kiel, Biotest Aps 15

16 Alfalfa harvest Pauli Kiel, Biotest Aps 16

17 WP 2 Feed additives and protein extraction Harvest Wet separation in juice and pulp Separation in: Feed and food proteins Other product (Saponins and beta-carotene) Fermentation medium Pauli Kiel, Biotest Aps 17

18 Harvest fractionation of alfalfa The stripped leaves have a high protein content and can serve as raw material for processing into value-added products The sterm fraction has a high content of lignocellulosis can serve as biomass feedstock for further processing and production biochemicals, antimicrobial agents, food additives and bio-fuels Pauli Kiel, Biotest Aps 18

19 Harv Pauli Kiel, Biotest Aps 19

20 Havest of alfalfa leaves (stripping) K. Shinners, 2007 Pauli Kiel, Biotest Aps 20