Cigaretter. Bestemmelse af nikotin i røgkondensat. Gaskromatografisk metode.

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1 Dansk Standard DS/ISO udgave Godkendt: Cigaretter. Bestemmelse af nikotin i røgkondensat. Gaskromatografisk metode. Cigarettes - Determination of nicotine in smoke condensates - Gas-chromatographic method DANSK STANDARD - Eftertryk uden tilladelse forbudt Kollegievej 6 DK-2920 Charlottenlund Tel (+45) Fax (+45) [email protected]

2 DS/ISO København DS Projekt: ICS: Deskriptorer: kemisk analyse og prøvning,tobaksprodukter,tobak,røgkondensat,nikotin,gaskromatografi,indholdsbestemmelse,cigaretter Første del af denne publikations betegnelse er: DS/ISO, hvilket betyder, at det er en international standard, der har status som dansk standard Denne publikations overensstemmelse er: IDT med: ISO 10315:2000 Denne publikation erstatter : DS/ISO 10315:1992 DS-publikationstyper DANSK STANDARD udgiver forskellige publikationstyper. Typen på denne publikation fremgår af forsiden. Der kan være tale om: Dansk standard = standard, der er udarbejdet på nationalt niveau, eller som er baseret på et andet lands nationale standard eller = standard, der er udarbejdet på internationalt og/eller europæisk niveau, og som har fået status som dansk standard DS-information = publikation, der er udarbejdet på nationalt niveau, og som ikke har opnået status som standard, eller = publikation, der er udarbejdet på internationalt og/eller europæisk niveau, og som ikke har fået status som standard, fx en teknisk rapport, eller = europæisk præstandard DS-håndbog = samling af standarder, eventuelt suppleret med informativt materiale DS-hæfte = publikation med informativt materiale Til disse publikationstyper kan endvidere udgives = tillæg og rettelsesblade DS-publikationsform Publikationstyperne udgives i forskellig form som henholdsvis = fuldtekstpublikation (publikationen er trykt i sin helhed) = godkendelsesblad (publikationen leveres i kopi med et trykt DS-omslag) = elektronisk (publikationen leveres på et elektronisk medie) DS-betegnelse Alle DS-publikationers betegnelse begynder med DS efterfulgt af et eller flere præfikser og et nr. fx DS 383, DS/EN 5414 osv. Hvis der efter nr. er angivet at A eller Cor, betyder det, enten at det er et tillæg eller et rettelsesblad til hovedstandarden, eller at det er indført i hovedstandarden. DS-betegnelse angives på forsiden. Overensstemmelse med anden publikation: Overensstemmelse kan enten være IDT, EQV, NEQ eller MOD = IDT: Når publikationen er identisk med en given publikation. = EQV: Når publikationen teknisk er i overensstemmelse med en given publikation, men præsentationen er ændret. = NEQ: Når publikationen teknisk eller præsentationsmæssigt ikke er i overensstemmelse med en given standard, men udarbejdet på baggrund af denne. = MOD: Når publikationen er modificeret i forhold til en given publikation.

3 INTERNATIONAL STANDARD ISO Second edition Cigarettes Determination of nicotine in smoke condensates Gas-chromatographic method Cigarettes Dosage de la nicotine dans les condensats de fumée Méthode par chromatographie en phase gazeuse Reference number ISO 10315:2000(E) ISO 2000

4 ISO 10315:2000(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In downloading this file, parties accept therein the responsibility of not infringing Adobe's licensing policy. The ISO Central Secretariat accepts no liability in this area. Adobe is atrademark of Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by ISO member bodies. In the unlikely event that aproblem relating to it is found, please inform the Central Secretariat at the address given below. ISO 2000 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office Case postale 56 CH-1211 Geneva 20 Tel Fax Web ISO 2000 All rights reserved

5 ISO 10315:2000(E) Foreword ISO (the International Organization for Standardization) is aworldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in asubject for which atechnical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 %of the member bodies casting avote. Attention is drawn to the possibility that some of the elements of this International Standard may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. International Standard ISO was prepared by Technical Committee ISO/TC 126, Tobacco and tobacco products. This second edition cancels and replaces the first edition (ISO 10315:1991), which has been technically revised. Annex Aof this International Standard is for information only. ISO 2000 All rights reserved

6 ISO 10315:2000(E) Introduction This International Standard may be considered as part of aset produced by ISO/TC 126 which describes the determination of total and nicotine-free dry particulate matter (NFDPM) in cigarette smoke condensates. The set comprises: ISO 3308, ISO 3402, ISO 4387, ISO 8243, ISO10315 and ISO A related International Standard, ISO 3400, determines total alkaloids, whereas this International Standard determines only nicotine by virtue of the gas-chromatographic separation. Occasionally, differences can occur because of minor amounts of alkaloids other than nicotine in some types of tobacco. Annex Aprovides information about the use of this method in conjunction with or simultaneously with the gaschromatographic method of water determination specified in ISO Abibliography is provided. ISO 2000 All rights reserved

7 INTERNATIONAL STANDARD ISO 10315:2000(E) Cigarettes Determination of nicotine in smoke condensates Gas-chromatographic method 1 Scope This International Standard specifies a method for the gas-chromatographic determination of nicotine in cigarette smoke condensates. The smoking of cigarettes and the collection of mainstream smoke are normally carried out in accordance with ISO However, the method specified in this International Standard is also applicable to the determination of nicotine in cigarette smoke condensates obtained by non-standard smoking. NOTE In countries not in a position to use the gas-chromatographic method, reference should be made to ISO 3400 for the determination of total nicotine alkaloids. In such cases, values obtained using the method described in ISO 3400 may be used with the addition of a note in the expression of results. 2 Normative references The following normative documents contain provisions which, through reference in this text, constitute provisions of this International Standard. For dated references, subsequent amendments to, or revisions of, any of these publications do not apply. However, parties to agreements based on this International Standard are encouraged to investigate the possibility of applying the most recent editions of the normative documents indicated below. For undated references, the latest edition of the normative document referred to applies. Members of ISO and IEC maintain registers of currently valid International Standards. ISO 4387, Cigarettes Determination of total and nicotine-free dry particulate matter using a routine analytical smoking machine. ISO 13276, Tobacco and tobacco products Determination of nicotine purity Gravimetric method using tungstosilicic acid. 3 Principle The smoke condensate from the mainstream smoke is dissolved in a solvent containing an internal standard. The nicotine content of an aliquot of the solution is determined by gas chromatography, and the nicotine content of the whole of the smoke condensate is calculated. 4 Reagents Use only reagents of recognized analytical reagent grade. 4.1 Carrier gas: helium or nitrogen of high purity. 4.2 Auxiliary gases: air and hydrogen of high purity for the flame ionization detector. 4.3 Propan-2-ol, with maximum water content of 1,0 mg/ml. 4.4 Internal standard: n-heptadecane or quinaldine (of purity at least 99 %). ISO 2000 All rights reserved 1