Phosphate LR L mg/l P Phosphomolybic Acid / Ascorbic Acid
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1 Phosphate LR L mg/l P Phosphomolybic Acid / Ascorbic Acid 334 Instrument specific information The test can be performed on the following devices. In addition, the required cuvette and the absorption range of the photometer are indicated. Instrument Type Cuvette λ Measuring Range MD 600, MD 610, MD 640 ø 24 mm 660 nm mg/l P XD 7000, XD 7500 ø 24 mm 660 nm mg/l P Material Required material (partly optional): Reagents Packaging Unit Part Number Phosphate LR Reagent Pack 1 pc. 56R KS278-Sulphuric Acid 50 % V/V 65 ml 56L KS135 Pa1/Alk1-Phenolphthalein Sub-Alk P 65 ml 56L KS144-CH2-FC4-Calcium Hardness Buffer 65 ml 56L KP962-Ammonium Persulphate Powder Powder / 40 g 56P The following accessories are required. Accessory Packaging Unit Part Number Stirring rod and spoon 1 pc. 56A Application List Waste Water Treatment Boiler Water Drinking Water Treatment Raw Water Treatment Pool Water Control NL EN Handboek Handbook van of Methoden Methods 01/19
2 Preperation 1. Strongly buffered samples or samples with extreme ph values should be adjusted to between ph 6 and ph 7 before the analysis (use 1 mol/l Sulphuric acid or 1 mol/l Sodium hydroxide). 2. Prior digestion is required for the analysis of Polyphosphate and total phosphate. Notes 1. The measuring spoon supplied with the reagents must be used for the correct dosage. 2. The long scoop is for KP962. The short scoop is for KP119. NL EN Handboek Handbook van of Methods Methoden 01/19 01/19
3 Digestion total Phosphate LR with liquid reagents 15 Fill a suitable digestion vessel with 50 ml homogenised sample. 20 min Add a measuring scoop KP962 (Ammonium Persulfate Powder). Add 15 drops KS278 (50% sulfuric acid) Boil the sample for 20 minutes. A sample volume of about 25 ml should be retained; If necessary, fill with deionised water. Invert the vial and allow to cool to room temperature. 50 ml Add 2 drops KS135 (Phenolphthalein Substitute Indikator). Add KS 144 (Calcium Hardness Buffer) drop by drop to the same sample until colouration turns from light pink to red. (Note: make sure to swirl the vial after adding each drop!) Fill the sample with deionised water to 50 ml. NL EN Handboek Handbook van of Methoden Methods 01/19
4 Digestion Polyphosphate LR with liquid reagents min Fill a suitable digestion vessel with 50 ml homogenised sample. Add 15 drops KS278 (50% sulfuric acid). Boil the sample for 20 minutes. A sample volume of about 25 ml should be retained; If necessary, fill with deionised water. 2 Invert the vial and allow to cool to room temperature. 50 ml Add 2 drops KS135 (Phenolphthalein Substitute Indikator). Add KS 144 (Calcium Hardness Buffer) drop by drop to the same sample until colouration turns from light pink to red. (Note: make sure to swirl the vial after adding each drop!) Fill the sample with deionised water to 50 ml. NL EN Handboek Handbook van of Methods Methoden 01/19 01/19
5 Implementation of the provision Phosphate LR with fluid reagent Select the method on the device For this method, no ZERO measurements are to be carried out with the following devices: XD 7000, XD ml 10 ml 24 mm Filter approx. 14 ml sample with a pre-rinsed filter (pore size 0.45 µm). Fill 24 mm vial with 10 ml prepared sample. Close vial(s). Zero Sample Place sample vial in the sample chamber. Pay attention to the positioning. Press the ZERO button. For devices that require no ZERO measurement, start here. 50 Remove the vial from the sample chamber. Hold cuvettes vertically and add equal drops by pressing slowly. Add 50 drops KS80 (CRP). Close vial(s). NL EN Handboek Handbook van of Methoden Methods 01/19
6 Invert several times to mix the contents. Add a measuring scoop KP119 (Ascorbic Acid). Close vial(s). Test Sample Swirl around to dissolve the powder. 10 min Place sample vial in the sample chamber. Pay attention to the positioning. Press the TEST (XD: START) button Wait for 10 minute(s) reaction time. Once the reaction period is finished, the measurement takes place automatically. The result in mg/l Phosphate appears on the display. NL EN Handboek Handbook van of Methods Methoden 01/19 01/19
7 Implementation of the provision Polyphosphate LR with liquid reagents Select the method on the device For this method, no ZERO measurements are to be carried out with the following devices: XD 7000, XD 7500 For testing of Polyphosphate LR with liquid reagents, carry out the described digestion. This test determines the content of inorganic total phosphate. The Polyphosphate content arises from the difference between inorganic and ortho phosphate. The test for Polyphosphate LR with liquid reagents runs just as the test under Method 334, Phosphate LR with liquid reagents. The result in mg/l anorganic Total Phosphate (ortho-phosphate and Polyphosphate) appears on the display. NL EN Handboek Handbook van of Methoden Methods 01/19
8 Implementation of the provision total Phosphate LR with liquid reagent Select the method on the device For testing of total Phosphate LR with liquid reagents, carry out the described digestion. This test determines all compounds of phosphorus present in the sample, including ortho-phosphate, polyphosphate, and organic phosphorus compounds. The test for total Phosphate LR with liquid reagents runs just as the test under Method 334, Phosphate LR with liquid reagents. The result in mg/l total Phosphate appears on the display. NL EN Handboek Handbook van of Methods Methoden 01/19 01/19
9 Analyses The following table identifies the output values can be converted into other citation forms. Unit Cite form Scale Factor mg/l P 1 mg/l 3- PO mg/l P 2 O Chemical Method Phosphomolybic Acid / Ascorbic Acid Appendix Interferences Persistant Interferences Large amounts of unresolved substances can cause non-reproducible measurement results. Interference from / [mg/l] Al AsO 4 in all quantities Cr 100 Cu 10 Fe 100 Ni 300 SiO 2 50 Si(OH) 4 10 S 2- in all quantities Zn 80 According to DIN ISO D49 Standard Method 4500-P E US EPA a) determination of free, combined and total b) Reactor is necessary for COD (150 C), TOC (120 C) and total -chromium, - phosphate, -nitrogen, (100 C) c) MultiDirect: Adapter is necessary for Vacu-vials (Order code ) d) Spectroquant is a Merck KGaA Trademark e) alternative reagent, used instead of DPD No.1/No.3 in case of turbidity in the water sample caused by high concentration of calcium and/or high conductivity f) additionally required for determination of bromine, chlorine dioxide and ozone in the presence of chlorine g) Reagent recovers most insoluble iron oxides without digestion h) additionally required for samples with hardness values above 300 mg/l CaCO 3 i) high range by dilution # including stirring rod, 10 cm NL EN Handboek Handbook van of Methoden Methods 01/19
10 NL EN Handboek Handbook van of Methods Methoden 01/19 01/19
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