Dansk standard DS/ISO 15685 1. udgave 2004-04-19 Jordundersøgelser Bestemmelse af mulig salpeterdannelse i jord Hurtigprøve ved ammoniumoxidering Soil quality Determination of potential nitrification Rapid test by ammonium oxidation
DS/ISO 15685 København DS projekt: 41127 ICS: 13.080.30 Deskriptorer: ammoniumbestanddele,biologiske prøver,kemisk analyse og prøvning,kemikalier,jordsammensætning,definitioner, bestemmelse,laboratorieprøvning,laboratorieprøver,målinger,analysemetoder,salpeterdannelse,salpeterbakterier,nitrogen, hurtigmetode,jordanalyse,jordundersøgelser,jordprøver,jord,prøver,giftighed Første del af denne publikations betegnelse er: DS/ISO, hvilket betyder, at det er en international standard, der har status som dansk standard Denne publikations overensstemmelse er: IDT med: ISO 15685:2004. DS-publikationen er på engelsk. DS-publikationstyper DANSK STANDARD udgiver forskellige publikationstyper. Typen på denne publikation fremgår af forsiden. Der kan være tale om: Dansk standard standard, der er udarbejdet på nationalt niveau, eller som er baseret på et andet lands nationale standard eller standard, der er udarbejdet på internationalt og/eller europæisk niveau, og som har fået status som dansk standard DS-information publikation, der er udarbejdet på nationalt niveau, og som ikke har opnået status som standard, eller publikation, der er udarbejdet på internationalt og/eller europæisk niveau, og som ikke har fået status som standard, fx en teknisk rapport, eller europæisk præstandard DS-håndbog samling af standarder, eventuelt suppleret med informativt materiale DS-hæfte publikation med informativt materiale Til disse publikationstyper kan endvidere udgives tillæg og rettelsesblade DS-publikationsform Publikationstyperne udgives i forskellig form som henholdsvis fuldtekstpublikation (publikationen er trykt i sin helhed) godkendelsesblad (publikationen leveres i kopi med et trykt DS-omslag) elektronisk (publikationen leveres på et elektronisk medie) DS-betegnelse Alle DS-publikationers betegnelse begynder med DS efterfulgt af et eller flere præfikser og et nr. fx DS 383, DS/EN 5414 osv. Hvis der efter nr. er angivet at A eller Cor, betyder det, enten at det er et tillæg eller et rettelsesblad til hovedstandarden, eller at det er indført i hovedstandarden. DS-betegnelse angives på forsiden. Overensstemmelse med anden publikation: Overensstemmelse kan enten være IDT, EQV, NEQ eller MOD IDT: Når publikationen er identisk med en given publikation. EQV: Når publikationen teknisk er i overensstemmelse med en given publikation, men præsentationen er ændret. NEQ: Når publikationen teknisk eller præsentationsmæssigt ikke er i overensstemmelse med en given standard, men udarbejdet på baggrund af denne. MOD: Når publikationen er modificeret i forhold til en given publikation.
INTERNATIONAL STANDARD ISO 15685 First edition 2004-03-15 Soil quality Determination of potential nitrification and inhibition of nitrification Rapid test by ammonium oxidation Qualité du sol Détermination de la nitrification potentielle et inhibition de la nitrification Essai rapide par oxydation de l'ammonium Reference number ISO 15685:2004(E) ISO 2004
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ISO 15685:2004(E) Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. ISO 15685 was prepared by Technical Committee ISO/TC 190, Soil quality, Subcommittee SC 4, Biological methods. ISO 2004 All rights reserved
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INTERNATIONAL STANDARD ISO 15685:2004(E) Soil quality Determination of potential nitrification and inhibition of nitrification Rapid test by ammonium oxidation 1 Scope This International Standard specifies a rapid method for the determination of the potential rate of ammonium oxidation and inhibition of nitrification in soils. This method is suitable for all soils containing a population of nitrifying microorganisms. It can be used as a rapid screening test for monitoring soil quality and quality of wastes, and is suitable for testing the effects of cultivation methods, chemical substances and pollution in soils. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO 11465:1993, Soil quality Determination of dry matter and water content on a mass basis Gravimetric method 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 inhibitory dose ID amount of a chemical added to soil that effectively inhibits biological activity by a stated percentage after a given time, in comparison to an untreated control NOTE It is expressed as a percentage. For example, ID25 and ID50 indicate 25 % and 50 % inhibition of biological activity, respectively. 3.2 biosolid organic fertiliser used in agriculture, including sewage sludge, compost, manure and industrial products 4 Principle Ammonium oxidation, the first step in autotrophic nitrification in soil, is used to assess the potential activity of microbial nitrifying populations. Autotrophic ammonium-oxidizing bacteria are exposed to ammonium sulfate in a soil slurry buffered at ph 7,2. Chemical test substances, extracts of biosolids, as well as samples of polluted soils can be added to the slurry at various concentrations. Oxidation of the nitrite formed by nitrite-oxidizing bacteria in the slurry is inhibited by the addition of sodium chlorate. The subsequent accumulation of nitrite is measured over a 6-h incubation period, and is taken as an estimate of the potential activity of ammoniumoxidizing bacteria. As the generation time of ammonia-oxidizing bacteria is long ( > 10 h), the method provides a measure of the potential activity of the nitrifying population at the time of sampling. It does not measure growth of the nitrifying population. Nitrite can be measured quickly and accurately and hence the method is fast, accurate, reproducible and inexpensive. ISO 2004 All rights reserved 1