PCR & Molecular Biology. Liquid Handling

Transkript

1 PCR & Molecular Biology Liquid Handling

2 Your Sarstedt Team 2

3 Compatibility table Compatibility table

4 PCR Performance Tested Quality The Sarstedt quality and purity standard PCR Performance Tested DNA-free DNase/RNase-free PCR inhibitor-free Human DNA < 0.5 pg/μl Bacterial DNA < 0.02 pg/μl DNase < 1x10-5 U/μl RNase < 1x10-9 Kunitz units/μl Biosphere plus quality Unrivalled purity Biosphere plus Sterile DNA-free DNase/RNase-free PCR inhibitor-free ATP-free Non-pyrogenic /endotoxin-free Human DNA < 5.0 fg/μl Bacterial DNA < 0.2 fg/μl DNase < 5x10-7 U/μl RNase < 5x10-11 Kunitz units/μl ATP < 1x10-12 mmol/μl Pyrogens < EU/ml Sterility validated in accordance with ISO 11135

5 Conformity testing in accordance with the international test standard ISO

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7 Piston Pipette shaft Aerosol Sample Aerosol-free Filter

8 Tailored tip solutions for every application

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11 Tip: to easily identify which tip is suitable for your pipette, use the compatibility table on pages 18-21or at

12 loss (rel. units) A B SARSTEDT A B C D

13 Product (Illustrations: actual size) Tray color code Order no. Volume Purity Grade Packaging Type E Type E Type N Type J Type A Type A Type C Type L Type B Product Tray color code Type E Order no. Volume Packaging Type N Type J Type A Type L Type B

14 Simple and practical Empty 96-tip reload trays and bulk-bagged tips Suitable for manually racking bulk tips for autoclaving and convenient handling. Economical and environmentally friendly Tip StackPack packaging reducing packaging and storage space required in the Tip SystemBox Order no. Design Packaging Pcs./case Order no. Tray color code Tip type/volume Packaging Pcs./case

15 Order no. Volume Purity Grade Packaging

16 Filter tips (Illustrations: actual size) Tray color code Order no. Max. sample volume/color Purity Grade Packaging Type E Type E Type N Type J Type A Type A Type O Extra long illustration ion 1:2 Type C Type L Type B Type M illustration 1:2

17 Tips without filters (Illustrations: actual size) Tray color code Order no. Max. sample volume/color Purity Grade Packaging Type E Type N Type J Type A illustration 1:2 Type O Extra long Gelloader illustration 1:2 Type C Type L Type B Type M illustration 1:2 illustration 1:2 illustration 1:2 illustration 1:2

18 μl μl 2-20 μl 2-20 μl μl 100-1,000 μl μl 100-1,000 μl 1-10 ml μl ml 5 ml 10 ml = compatible 10 = limited pipetting volume = not recommended = not checked MC = multi-channel pipette

19 100-1,000 μl 1-10 ml μl μl μl Xplorer 100-1,000 μl 200-1,000 μl 1-10 μl μl 100-1,000 μl μl 100-1,000 μl Bio Control P10/U P100/U100 P200/U μl P1.000/U ,000 μl P5.000/U μl P10/U ml Xplorer Bio Control

20 μl μl 100-1,000 μl 100-5,000 μl μl μl 100-1,000 μl 100-5,000 μl μl 50-1,000 μl μl ml 5 ml 10 ml = compatible 10 = limited pipetting volume = not recommended = not checked MC = multi-channel pipette

21 A. 835/ 935e μl 2-20 μl 2-20 μl μl 100-1,000 μl μl μl μl 100-1,000 μl 1-10 ml μl 100-1,000 μl 1-10 μl 1-10 μl 2-20 μl μl 100-1,000 μl 1-10 μl μl μl μl 1-10 μl 2-20 μl 100-1,000 μl ml A. 835/ 935e

22 Can be opened without touching the inside of the lid 22

23 Product Order no. Vol. Color Quality Grade Units/bag Units/case Product Order no. Vol. Profile Color Lid strip Quality Grade Units/bag Units/case Product Order no. Multiply μstrip, suitable for Color Quality Grade Units/bag Units/case

24 Product Order no. Product description Design Packaging unit Order no. Capacity Format Suitable for Packaging

25 t White wells C t values for the detection of plasmid copies in white and transparent plates at reduced reaction volumes Fluorescence C t values Transparent wells Cycles Number of plasmid copies C t C t Fluorescence level 10x higher Greater sensitivity Improved reproducibility

26 Multiply Tip: to easily identify which plate is suitable for your thermocycler use our compatibility table on page 30/31 or at Order no. Color Skirt Deck Profile Well Max. volume Units/bag Units/case Order no. Color Skirt Deck Profile Well Max. volume Units/bag Units/case Order no. Color Skirt Deck Profile Well Max. volume Units/bag Units/case

27 Order no. Color Skirt Deck Profile Well Max. volume Units/bag Units/case Order no. Color Skirt Deck Profile Well Max. volume Blister packs/box Order no. Colour Skirt Deck Profile Well Max. volume Units/bag Units/case

28 Order no. Color Skirt Deck Profile Well Max. volume Units/bag Units/case Order no. Color Skirt Deck Profile Well Max. volume Units/bag Units/case Product Order no. Description Design Color Units/bag Units/case Order no. Color Skirt Deck Well Max. volume Units/bag Units/case

29 Order no. Color Skirt Deck Well Max. volume Units/bag Units/case

30 Number of wells 96 pre-inserted full skirt Half/full skirt Without Without Half Full Half Full High High High Low Low - Order no. PCR plates Amersham Biosciences / GE Healthcare Analytik Jena /Biometra 2 2 Applied Biosystems /Life Technologies Veriti Veriti PeqLab

31 Number of wells 96 pre-inserted full skirt 96-well 384-well Half/full skirt Without Without Half Full Half Full Full Full High High High Low Low - Lightcycler Lightcycler Order no. PCR plates BioRad /MJ Research Corbett Research /Qiagen Eppendorf Ericom MWG Roche Stratagene /Agilent Techne

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33 Order no. Product description Application Special properties Clear Pierceable Functional temperature range Packaging Film/foil properties Order no.

34 Standard tube Tubes with low binding properties BSA loss (%) Sarstedt Supplier

35 Experiment method: Fluorescence Cycle Sarstedt Control A B C D Control Sarstedt Supplier A Supplier B Supplier C Supplier D Volume Order no. Description Packaging Volume Order no. Purity Grade Packaging

36 Order no. Design Volume/material Units/bag Units/case Order no. Design Units/bag Units/case

37 Wavelength Transmission (T) in % (nm) UV Acrylic Polystyrene Order no. Height in mm LCH Clear sides Units/Styrofoam rack/case

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40 Revolver Workstation SARSTEDT AG & Co. P.O. Box D Nümbrecht Phone Fax /bag 4/case 1 Microbiology Sample collection, cultivation, processing Screw Cap Micro Tubes Transport, storage and sample preparation Inner sub-heading 14pt Cell and tissue culture Cell Cultivation, Cryogenic Storage, Filtration, Liquid Handling Revolver Workstation Designed to accommodate a range of tube sizes in one rack Units are rotatable and freely exchangeable Ideal for use in the temperature range from -80 C to 121 C L x W x H (per unit): 108 x 108 x 257 (108 x 108 x 69) mm Ordering Information Art. No. Description Packaging IVARO Tube Handler XS Innovative automation for tube processing ELISA Plates Micro Test Plates for Immunoanalytics