Supplemental Table 1: Known proteins of plant peroxisomes identified in isolated leaf peroxisomes from Arabidopsis.

Transkript

1 Supplemental Table 1: Known proteins of plant peroxisomes identified in isolated leaf peroxisomes from Arabidopsis. Acronym Protein Gene locus Size (aa; MW) pi Approach of protein identification PTS1/2 Gelbased Shotgun Photorespiration GOX1 Glycolate oxidase isoform 1 At3g ; yes yes PRL> GOX2 Glycolate oxidase isoform 2 At3g ; yes yes ARL> SGT Serine-glyoxylate aminotransferase At2g ; yes yes SRI> HPR Hydroxypyruvate reductase At1g ; yes yes SKL> GGT1 Glutamate-glyoxylate aminotransferase isoform 1 At1g ; yes yes SKM GGT2 Glutamate-glyoxylate aminotransferase isoform 2 At1g ; yes yes SRM pmdh1 Perox. malate dehydrogenase isoform 1 At2g ; yes yes RIx HL pmdh2 Perox. malate dehydrogenase isoform 2 Atg ; yes yes RIx HL ROS metabolism CAT1 Catalase isoform 1 At1g ; n.d. yes (mpts) CAT2 Catalase isoform 2 At4g300 42; yes yes (mpts) CAT3 Catalase isoform 3 At1g ; yes yes (mpts) APX3 Perox. ascorbate peroxidase isoform 3 At4g ; yes yes mpts MDAR1 Monodehydroascorbate reductase isoform 1 At3g ; yes n.d. AKI> CSD3 Cu/Zn superoxide dismutase Atg ; yes yes AKL> Core fatty acid β-oxidation LACS7 Long-chain acyl-coa synthetase isoform 7 Atg ; yes n.s. 2 SKL> ACX4 Acyl-CoA oxidase isoform 4 At3g ; yes yes SRL> AIM1 Abnormal fluorescence meristem 1 At4g ; yes yes SKL> MFP2 Multifunctional protein 2 At3g ; n.d. yes SRL> PED1/KAT2 Peroxisome Defective 1 At2g ; yes yes RQx HL PKT1/KAT1 Thiolase isoform 1 At1g ; n.d. yes RQx HL PKT2/KAT Thiolase isoform 2 Atg ; yes yes RQx HL Glyoxylate cycle CSY3 Citrate synthase 3 At2g4270 0; yes yes RAx HL ASP3 Aspartate aminotransferase Atg ; n.d. yes RIx HL Auxiliary β-oxidation enzymes DCI Δ 3, -Δ 2,4 - dienoyl-coa isomerase Atg ; yes n.d. AKL> ECH2 Enoyl-CoA hydratase 2 At1g ; yes n.s. 2 SSL> SCP-2 Sterol carrier protein isoform 2 Atg ; yes yes SKL> ACH2 Acyl-CoA thioesterase At1g ; yes n.d. SKL> AAE7/ACN1 Acyl-activating enzyme isoform 7 At3g1610 6; n.d. yes SRL> JA biosynthesis OPR3 Oxophytodienoic acid isoform 3 At2g ; yes yes SRL> 4CLP1 4-Coumarate-CoA ligase-like protein 1 At4g ;. 8.6 n.d. yes SKM> OPCL1 OPC-8:0 ligase 1 At1g ;.4 8. yes n.d. SKL>

2 N- and S- metabolism URI Uricase At2g ; yes yes SKL> SOX Sulfite oxidase At3g ; n.d. yes SNL> Chaperones ACD31.2-Px α-crystallin domain containing protein of 31.2 kda At1g ; yes yes RLx HF Biogenesis proteins PEX11d PEX11d At2g ; yes n.d. n.d. PEX14 PEX14 Atg ; n.d. yes n.d. For the 36 known plant peroxisomal proteins identified in this study, acronym, gene locus, PTS1/2 or internal PTS (mpts, e.g. CAT1-3), polypeptide size of the longest variant (in amino acid residues, aa, and molecular weight, MW), isoelectric point (pi), additional information on predicted targeting signals, and the method used for protein identification (2-D gel based or shotgun approach) are provided. The values for MW and pi were calculated by EXPASY ( When proteins were represented by only a single peptide in the shotgun approach, it is indicated whether this was sufficient (yes 1 ) or not significant (n.s. 2 ) for protein identification. Transmembrane domains (TMD) were predicted according to Lee et al. (2006, localizome.org/) and Krogh et al. (2001, services/tmhmm/). n.d., not detected; n.s., not significant References: Krogh, A., Larsson, B., von Heijne, G., and Sonnhammer, E. L. L. (2001) Predicting transmembrane protein topology with a hidden Markov model: Application to complete genomes. J. Mol. Biol. 30: Lee, S., Lee, B., Jang, I., Kim, S., and Bhak, J. (2006b). Localizome: a server for identifying transmembrane topologies and TM helices of eukaryotic proteins utilizing domain information. Nucl. Acids. Res.34: W-103.

3 Supplemental Table 2. MS data of proteins identified. Acronym Gene locus Size (aa; MW) pi Gel-based approach Shotgun approach PMF Sequence Coverage (%) PMF Peptide sequenced MS/MS Peptides Score score a ion score b 4CLP1 At4g ; K.DPNTSLVSFLFR.N K.LAIADSDTGDSLTFSQLK.S PGDH At3g ; WTVQQAAELSVPAPTIESSLDAR 32 AAE Atg ; FSYTYDDLIHR 4 TVGFTEIDVVDPESGR 3 AAE7/ACN1 At3g1610 6; K.IPANYTALTPLWFLDR.A R.YTGM*EQLDVIDTQTGKPVPADGK.T K.ETFAGGWFHSGDIAVK.H ACAT2 Atg ; GIAAQEAGAFTWEIVPVEVSGGR EQQDDYAVQSFER ACD31.2 At1g ; DNVSAEFMDGILR QASSAQGFFMR 1 22 K.LANLGTVWSPR.S R.SNVAESTHSYVVAIELPGASINDIR.V K.DNVSAEFM*DGILR.I ACH2 At1g ; ILQLDPLELNIFR 2 ACX4 At3g ; ELLGGNGILADFLVAK AFCDLEPIYTYEGTYDINTLVTGR 8 30 R.ELLGGNGILADFLVAK.A R.EVTGIASFKPATR.S AIM1 At4g ; AQLGLPELTLGVIPGFGGTQR FSGGFDINVFQQVHK R.FSGGFDINVFQQVHK.T K.AQLGLPELTLGVIPGFGGTQR.L K.LGLIDALVPPGDVLSTSR.K R.IVGAHFFSPAHLM*PLLEIVR.S K.LSETYGSFFKPSR.Y APX3 At4g ; MGLSDKDIVALSGGHTLGR FDNSYFVELLK K.FDNSYFVELLK.G K.LSELGFNPNSSAGK.A ASP3 Atg ; R.TEEGKPLVLNVVR.K 2.04

4 K.EYLPIVGLVEFNK.L K.TIYITQPTWGNHPK.I ATF1 At1g ; FETDDHEAFIEYK IVWNEGR BADH At3g ; BGL1 At1g ; NEGATVLCGGVRPEHLK R.QLFIGGQWTEPVLR.K e) 3.13 CENHNADVAVDFYHR CSPYIPGYGQHCQDGR 8 68 K.VGVQFYHDLIDELLK.N K.HWITFNEPWVFSR.A R.SGYEAYQVSHNLLLSHAYAVDAFR.N K.GSTDYVGM*NYYTSVFAK.E BSMDR/ARP At1g ; K.IIYAGVNASDVNFSSGR.Y d) 4.1 CAT1 At1g ; R.GPILLEDYHLLEK.L R.GPGVQTPVIVR.F R.EGNFDLVGNNFPVFFVR.D K.FPDM*VHALKPNPK.S K.SHIQENWR.I K.IWPEDILPLQPVGR.L R.LGPNYLQLPVNAPK.C R.SIWISYWSQADK.S CAT2 At4g300 42; EGNFDLVGNNFPVFFIR GFFEVTHDISNLTCADFLR TWPEDILPLQPVGR R.GPILLEDYHLVEK.L R.EGNFDLVGNNFPVFFIR.D R.HM*DGSGVNTYM*LINK.A R.VGGTNHSHATQDLYDSIAAGNYPEWK.L K.TWPEDILPLQPVGR.M CAT3 At1g ; EGNFDLVGNNTPVFFIR GFFEVTHDISNLTCADFLR K.YRPSSAYNAPFYTTNGGAPVSNNISSLTIGER.G R.GPVLLEDYHLIEK.V R.APGVQTPVIVR.F R.EGNFDLVGNNTPVFFIR.D K.TNIQEYWR.I R.HM*EGFGVHTYTLIAK.S K.VPTPTNSYTGIR.T K.RWVEILSEPR.L R.WVEILSEPR.L R.GIWISYWSQADR.S R.LNVRPS.I CSD3 Atg ; HAGDLGNILAGSNGVAEILIK HIPLSGQYSILGR 10 1 K.HIPLSGQYSILGR.A R.HAGDLGNILAGSNGVAEILIK.D R.AVALIAGDNNVR.G CSY3 At2g4270 0; LYPNVDFYSGLIYR LYDPGYLNTAPVR K.APTIAAAAYLR.M K.LYPNVDFYSGLIYR.A

5 DCI Atg ; EVDLAIVADLGTLQR LPSIVGYANAMELALTAR ECH2 At1g ; LSGDYNPLHSDPEFAK 31 K.TVLAGYVDIR.G e) 3.12 ECHIa At4g ; R.FGIFPSWGLSQK.L R.EVSLTSM*PLTADVAGK.L ECHIb At4g ; FHPDTIATILSLLEQAK SLYPELCGILGLETR R.FHPDTIATILSLLEQAK.S K.FFSNGFDLAWAQTAGSK.T ECHIc At1g ; LNPTLLDSLR EVLIHTIGEYESGSSVVR 4 3 EH3 At4g ; VNALVNTSVVFNPR ALFGDDYYICR 8 66 ESM1 At3g ; ANPNADASAQQAFVTNVINR SYFFFDGR GGT1 At1g ; AMVIINPGNPTGQCLSEANIR HYLSLTSGGLGAYSDSR K.ALDYDTLNENVK.K K.KIIFTNVGNPHALGQKPLTFPR.Q K.IIFTNVGNPHALGQK.P K.IIFTNVGNPHALGQKPLTFPR.Q K.HYLSLTSGGLGAYSDSR.G R.DGYPSDPELIFLTDGASK.G K.LVLLGDEVYQQNIYQDER.P K.LVLLGDEVYQQNIYQDERPFISSK.K K.KVLM*EM*GSPFSK.E K.VLM*EM*GSPFSK.E K.EVQLVSFHTVSK.G R.GGYFEM*TNLPPR.V R.LPTGALQAAK.Q K.LLEATGISTVPGSGFGQK.E R.TTILPAEDEM*PEIM*DSFK.K R.TTILPAEDEM*PEIM*DSFKK.F K.FNDEFM*TQYDNNFGYSK.M GGT2 At1g ; AMVIINPGNPTGQCLSEANIR HYLSLTSGGLGAYSDSR K.EVQLISFHTVSK.G R.GGYFEM*TNIPPR.T K.FNDEFM*SQYADNFGYSR.M GOX1 At3g ; IAIQAGAAGIIVSNHGAR VPVFLDGGVR M*EITNVTEYDAIAK.A K.M*VYDYYASGAEDQWTLQENR.N R.ILIDVNKIDM*ATTVLGFK.I K.IDM*ATTVLGFK.I K.ISM*PIM*VAPTAFQK.M K.M*AHPDGEYATAR.A

6 GOX2 At3g ; R.AASAAGTIM*TLSSWATSSVEEVASTGPGIR.F R.FFQLYVYK.N IAIQAGAAGIIVSNHGAR 138 R.ILIDVSKIDM*TTTVLGFK.I K.IDM*TTTVLGFK.I K.ISM*PIM*VAPTAM*QK.M K.M*DEANDSGLASYVAGQIDR.T R.TLSWKDVQWLQTITK.L K.DVQWLQTITK.L K.DVQWLQTITKLPILVK.G R.QLDYVPATISALEEVVK.A R.IPVFLDGGVR.R R.IPVFLDGGVRR.G K.ALALGASGIFIGR.P R.PVVFSLAAEGEAGVR.K R.NHITTEWDTPR.P R.NHITTEWDTPRPSAR.L GOX3 At4g ; R.ATSAAGTIMTLSSWATCSVEEVASTGPGIR.F d) 2.26 GR At3g ; GVNLHPQTSLTQLTK TNIPSIWAVGDATNR 4 22 GRP7 At2g ; CFVGGLAWATDDR 7 R.ALETAFAQYGDVIDSK.I R.GFGFVTFKDEK.A R.SITVNEAQSR.G R.EGGGGYGGGEGGGYGGSGGGGG.W GRP8 At4g ; SGGGGGYSGGGGGGYSGGGGGGYE R 77 R.TFSQFGDVIDSK.I R.VITVNEAQSR.G R.EGGGYGGGDGGSYGGGGGG.W GSTT1 Atg ; K.SLSTLETFWLK.G K.ATMPHFDETHEILFK.V HBCDH At3g120 24; TTVCSQDYAGFVVNR 4 n.d HMGDH Atg ; VEPGSNVAIFGLGTVGLAVAEGAK AAVAYEPNKPLVIEDVQVAPPQAGEVR n.d. HPR At1g ; TILSVEDIIDLIGDK YGIAVGNTPGVLTETTAELAASLSLAAA R K.PVSIEVYNPNGK.Y K.AFSNM*AVGYNNVDVEAANK.Y K.YGIAVGNTPGVLTETTAELAASLSLAAAR.R R.IVEADEFM*R.G R.GGLYEGWLPHLFVGNLLK.G R.M*M*VEGFK.M K.M*NLIYFDLYQSTR.L K.FVTAYGQFLK.A R.EADLISLHPVLDK.T R.GPVIDEAALVEHLK.E R.GPVIDEAALVEHLKENPM*FR.V

7 R.VGLDVFEEEPFM*K.P R.VGLDVFEEEPFM*KPGLADTK.N K.NAIVVPHIASASK.W R.EGM*ATLAALNVLGR.V K.GYPIWHDPNR.V K.ALGLPVSK.L IDH At1g ; LIFPFLELDIK YFDLGLPNR 40 1 LACS7 Atg ; YEHLGQLCNDPR LIVVVGGADEHLPSLPR K.AVTLVPEPFTLENGLLTPTFK.I e) 2.83 MDAR1 At3g ; METE1 Atg ; YQTLIIATGSTVLR 38 YGAGIGPGVYDIHSPR GGIGVIQIDEAALR MFP2 At3g ; n.d. n.d. R.FSGGFDISGFGEM*QK.G R.ISAPAAQLGLPELQLGVIPGFGGTQR.L K.ALEM*ILTSKPVK.A R.IVGAHFFSPAHIM*PLLEIVR.T MIF At3g ; ELIATVGSILHTHFSIHPTR 103 NBP At4g ; R.HYLVIPK.E K.EHIPTVNDLQR.R NS/ECHId At1g ; LNVLDLQVQIR 7 K.VGSFDAGYGSSIM*SR.L K.M*GLINTVVPLEDLEK.E OASS A1 At4g ; YLSTVLFDATR LFVAIFPSFGER 36 1 OPCL1 At1g ; TPIPLPPNPSLDVTTFISSQAHR TGDLCYIDEDGFIFVVDR OPR3 At2g ; ALNGVPNAALAEYYAQR AGFDGIEIHGAHGYLIDQFLK R.AGFDGIEIHGAHGYLIDQFLK.D K.QVVEGVVSAIGASK.V R.M*AYNGTFM*SSGGFNK.E K.TFYTQDPVVGYTDYPFLAPFSR.L OZI1 At4g ; R.VAGPPVVMNPISR.Q R.VAGPPVVM*NPISR.Q PED1/KAT2 At2g ; TNLNPSEVGDIVVGTVLAPGSQR GLPVLGVFR R.TSLYGDDVVIVAAHR.T R.GNFKDTYPDDLLAPVLR.A K.DTYPDDLLAPVLR.A

8 K.TNLNPSEVGDIVVGTVLAPGSQR.A R.MAAFYAGFPETVAVR.T R.M*AAFYAGFPETVAVR.T K.AGFYDIGIGAGLESMTTNPMAWEGSVNPAVK.K K.GLPVLGVFR.T R.TFAAVGVDPAIMGIGPAVAIPAAVK.A K.INVNGGAMAIGHPLGATGAR.C PEX11d At2g ; FVNDLHGLISPVPK AELALVVMYLNK YQDEDYR PEX14 Atg ; R.VSQEMMITK.N K.IYSADQEVYNGSVTTAR.K K.SYMDIMSMIQR.G R.STDFGYETTTAAR.F R.FTANQNETSTMEPAAFQR.Q R.SWVPPQPPPVAMAEAVEAIR.R PKT1/KAT1 At1g ; K.DTFPDELLASVLR.A K.TNVNPSEVGDIVVGTVLGPGSQR.A PKT2/KAT Atg ; VNVNGGAIAIGHPLGATGAR VAAYFAGFPDSVPVR 0 4 K.DTLPDDLLASVLK.A R.AGYYDIGIGAGVESM*STDHIPGGGFHGSNPR.A K.VNVNGGAIAIGHPLGATGAR.C pmdh1 At2g ; DDLFNINAGIVR CGIDEVYGLGPLNEYER 74 8 K.VAILGAAGGIGQPLAMLM*K.M R.GFLGQPQLEEALTGM*DLVIIPAGVPR.K K.AIVNIISNPVNSTVPIAAEVFK.K K.AIVNIISNPVNSTVPIAAEVFKK.A K.LM*GVTM*LDVVR.A R.IQNGGTEVVEAK.A pmdh2 Atg ; TGAEEVYQLGPLNEYER ANTFVAEVLGLDPR K.VAILGAAGGIGQSLSLLM*K.M K.QLEDALTGMDLVIIPAGIPR.K K.QLEDALTGM*DLVIIPAGIPR.K K.CCPNAIVNLISNPVNSTVPIAAEVFK.K K.CCPNAIVNLISNPVNSTVPIAAEVFKK.A K.LLGVTTLDVAR.A R.ANTFVAEVLGLDPR.E K.PPSSFTPQEIEYLTNR.I K.AGAGSATLSMAYAAAK.F K.AGAGSATLSM*AYAAAK.F R.TGAEEVYQLGPLNEYER.I K.AKDELAGSIQK.G PYK10 At3g ; GVSQAGVQFYHDLIDELIK 62 R.SGYEAYLVTHNLLISHAEAVEAYR.K d).3 6 RBP At4g ; DGGAAAQAPTPAIGDSAQFPTLGK 81

9 NNDGPANENGYGGGYR 7 RH11 At3g ; SGIATAFFNENNAQLAR VVVAYGGTPIHQQLR RH37 At2g ; VVVAYGGTPINQQLR LAADFLANYIFLAVGR 21 c) 21 SCP-2 Atg ; KLGFEEVTYIVDLK IGLVYQINIAPK 86 K.IGLVYQINIAPK.K K.VDATFSFKDDDFVK.V SDRa At4g030 24; VAIVTASTQGIGFGITER VNAVAPGFVPTHFASFITGSSEVR R.FGLEGASVVVSSR.K R.VNAVAPGFVPTHFASFITGSSEVR.E SDRb/(DECR At3g ; SLGIQAIGLEGDVR GQVALITGGGSGIGFEISSQFGK 28 8 K.HGASIAIM*GR.R e) 2.3 SDRc At3g ; VQDILQVSQDEFHR ALYPGADAYASTSAAIHQLVR 4 K.LVM*M*GNEGSLR.S e) 2.63 SDRd At3g20 280; GGSVINISSIAGIR IDALINNAGIR 3 14 SGT At2g ; AALDLIFEEGLENIIAR HHLFVPGPVNIPEPVIR R.HHLFVPGPVNIPEPVIR.A R.SPAIPALTK.T K.TTSGTPFLFPTTGTGAWESALTNTLSPGDR.I R.M*DEWGVDVALTGSQK.A K.VFFDWNDYLK.F K.LGTYWPYTPSIQLLYGLR.A K.EEWISNTVTAVM*VPPHIDGSEIVR.R R.YNLSLGLGLNK.V SOX At3g ; R.SALVSSYVTPVDLFYK.R K.LADVLELVGIPK.L K.ASITLSQATNPEADVLLAYEM*NGETLNR.D K.AVDSAANVQPENVESVWNLR.G R.GVLNTSWHR.V ST2 At1g0420 1; IPPHLLNAGK 41 TGG1 Atg ; GFIFGVASSAYQVEGGR NWITINQLYTVPTR 3 13 TGG2 Atg280 47; GINEDGINYYSGLIDGLIAR 3 TLP Atg ; SGPLMDLVDALNPGTYR SRPPITTHVLDVSR 3

10 UP1 At1g2410 7; ELSHLLADASEAYEEYHGAVR LEFEAHHNSINQAGSDGVK 88 4 UP2 At3g10 772; DLVNHPNFDAIIAAGTR FTSLTTSLSELLK 7 66 UP3 At2g ; FPGIDQITVGENFSPAR EDLASYAAHPDHVR ISSATAFTHVLHSR UP4 At1g ; AGGFPEPEIVHYNPK SSLSAEGFDVVYDINGR 4 48 URI At2g ; FKDDVYLPTDEPHGSIEATLSR VLMDTFFGPPETGVYSPSVQR 8 3 K.SGALNLTSGIGGLALLK.T K.FKDDVYLPTDEPHGSIEATLSR.I a) Mascot protein score obtained for the peptide mass fingerprint (PMF). The significance threshold was 60. b) Mascot MS/MS ion scores obtained for the individual peptides sequenced. The significance threshold was 2-32 depending on how many peptides fell within the mass tolerance window about the precursor mass. Only the top ranking peptides matching a query for the first time ("bold red hits") are reported. c) RH37 was accepted as identified on the basis of two peptide matches indicating at least significant homology (threshold 21) though not identity (threshold 2). d, e) When proteins were represented by only a single peptide in the shotgun approach, it is indicated whether this was, upon manual inspection, significant ( d) ) or not significant ( e) ) for protein identification. In the shotgun peptides oxidized methionines within are labelled by an asterics (*), and proteolytic peptide cleavage sites with a dot, indicating fully or incomplete tryptic peptides. Isoform-specific peptides of peroxisomal isoforms sharing high sequence similarity are underlined to indicate specific identification of the respective protein isoform. n.d., not detected

11 Supplemental Table 3. Sequence conservation of SDR domains in peroxisomal SDRs. Nucleotide binding region Predicted Co-factor binding site Active site residues Pattern 1 Pattern 2 Pattern 3 TGxxxGhG hnhxg GxhhxhSSh Yx[AS][ST]K SDRa At4g030 TAxxxGhG NADP (7 to 4) SDRb/DECR At3g12800 TGxxxGhG NADP (pos. 8 to 0) hnhxs SxhhxhTSh YxVTK hdhxg GxhhxhSAT VxAAK SDRc At3g0180 n.d. n.d. hnhxa GxhhxhATh YxSTS SDRd At3g20 TGxxxGhG NADP (pos. 16 to 8) BSMDR/ARP At4g030 TGxxxGhG NAD (pos. 2 to 44) hnhxg GxhhxhSSh YxCSK hdhxa GxhhxhGSh YxASK The sequences of five peroxisomal SDRs were aligned using kalign (Lassmann and Sonnhammer, 200) and the conservation of active site residues involved in co-factor and substrate binding determined as compared to classical SDR motifs (Kallberg et al., 2002). Co-factor prediction was performed by the general Rossmann fold predictor ( Kallberg and Persson, 2006). The amino acid residues of the catalytic triad (Ser, Tyr, Lys) are underlined. Amino acid residues altered in peroxisomal SDRs are shaded gray. References: Kallberg, Y., Oppermann, U., Jornvall, H., and Persson, B. (2002). Short-chain dehydrogenases/reductases (SDRs). Eur. J. Biochem. 26: Kallberg, Y., and Persson, B. (2006). Prediction of coenzyme specificity in dehydrogenases/reductases. A hidden Markov model-based method and its application on complete genomes. FEBS J. 273: Lassmann, T., and Sonnhammer, E.L.L. (200). Kalign - an accurate and fast multiple sequence alignment algorithm. BMC Bioinformatics 6: 28.

12 Supplemental Table 4. Cloning, subcloning, and mutagenesis primers. Acronym Gene locus Vector with insert Primer ( to 3 ) 6PGL Atg PGL in pgemt for: CACCAAGACTGCGGCCGCTATGGCTACGACAAATATAGG rev: TGCTATCTAGAGCGGCCGCTTAGAGCTTGGAAGATGCTGG EYFP-PTD 6PGL in pcat rev: TATATCTAGATTAGAGCTTGGAAGATGCTGGTTTGTCCAGGAACTTGTACAGCTCGTCCATGCC 6PGDH At3g PGDH in pgemt for: CACCAAGACTGCGGCCGCTATGGCTGTTCAACCTACAAG rev: TGCTATCTAGAGCGGCCGCTCAGATCTTAGATTGTCTTGC EYFP-PTD 6PGDH in pcat rev: TATATCTAGATCAGATCTTAGATTGTCTTGCAATCTTGAACCACTTGTACAGCTCGTCCATGCC ATF1 At1g21770 ATF1 in pcat for1: TTAAATGGCGACGGGGACGGCGACGGAGAAACCGAAGATCGTGTGGAACGAAGGAAGAC for2: GAATAAGATTGCGGCCGCTATGGCGACGGGGACGGCGAC EYFP-PTD ATF1 in pcat rev: TGCTCTAGATCAGATGCTAGACTTGAGG rev: TATATCTAGAtcagatgctagacttgaggttcttggaatcatCCTTGTACAGCTCGTCCATGCC ECH2 At1g7610 ECH2 in pgemt for: AAGATTGCGGCCGCTATGGCGACTAGCGATTCTG ECHIb At4g14430 ECHIb in pgemt rev: TATATCTAGACTAAAGTGACGAAGATAAC for: AAGATTGCGGCCGCTATGTGTACGTTAGAGAAACG rev: TATATCTAGATTAGAGCTTAGGTGTTGCAAAC EYFP-PTD ECHIb in pcat rev: TATATCTAGATTAGAGCTTAGGTGTTGCAAACACTCTAGTCTCCTTGTACAGCTCGTCCATGCC EH3 At4g02340 EH3 in pgemt for: AAGATTGCGGCCGCTATGGAGAAGATAGAGCACAC rev: TATATCTAGACTACAAGGATGCGGTTTCGCG EYFP-PTD EH3 in pcat rev: TATATCTAGACTACAAGGATGCGGTTTCGCGGGTTCTGAATTTCTTGTACAGCTCGTCCATGCC GSTT1 Atg41210 GSTT1 in pgemt for: AAGATTGCGGCCGCTATGATGAAGCTCAAAGTG rev: TATATCTAGATTAGATCTTGGATTGAAGACCCGGTTTAGATAACTTGTACAGCTCGTCCATGCC EYFP-PTD GSTT1 in pcat rev: TATATCTAGATTAGATCTTGGATTGAAGACCCGGTTTAGATAACTTGTACAGCTCGTCCATGCC HBCDH At3g120 HBCDH in pgemt for: AAGATTGCGGCCGCTATGGCGGAGATGAAGAGTG rev: TATAGAGCTCTCAGAGCCGCGGAGACAGC EYFP-PTD HBCDH in pcat rev: TATATCTAGATCAGAGCCGCGGAGACAGCTTCTGTGTAGCTTCCTTGTACAGCTCGTCCATGCC IDH At1g4340 IDH in pgemt for: CACCAAGACTGCGGCCGCTATGGAATTCGAGAAGAT rev: TGCTATCTAGAGCGGCCGCTCACAACCTGGAGTTGTTGCCTA EYFP-PTD IDH in pcat rev: TATATCTAGATCACAACCTGGAGTTGTTGCCTAACAATCTTCTCTTGTACAGCTCGTCCATGCC EYFP-PTD ECH2 in pcat rev: TATATCTAGACTACAAGGATGCGGTTTCGCGGGTTCTGAATTTCTTGTACAGCTCGTCCATGCC

13 SDRb/DECR At3g1270 SDRb in pgemt for: AAGATTGCGGCCGCTATGGACTCTCCGTTCAAACC rev: TATATCTAGACTACAGCTTGCTGGTTGGG EYFP-PTD SDRb in pcat rev: TATATCTAGACTACAGCTTGCTGGTTGGGAGACCAACAGGCTTCTTGTACAGCTCGTCCATGCC TLP Atg8220 TLP in pgemt for: AAGATTGCGGCCGCTATGGCGATGGAGATCGGAG rev: TATATCTAGACTAGCTCCCACGGTATGTG EYFP-TLP(H/D) for: GATCGTCTGAGAATCATTGGAGGGGATTTGAATGTTGCAGCTGAAGCAAAAG rev: CTTTTGCTTCAGCTGCAACATTCAAATCCCCTCCAATGATTCTCAGACGATC