Supplemental Table 1. List of identified oxidatively modified plant proteins

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1 Supplemental Table 1. List of identified oxidatively modified plant proteins Protein (EC number) a) Species b) Acc. No. / ID c) Localization d) Modification e) Ref. f) protein GF14 ARATH NP_ Nitrosylation 1 Acidic ribosomal protein P2 (60S) ARATH NP_ Nitrosylation 1 Aconitase ORYSA gi Mit. Trp-oxidation 2 Aconitase ARATH Q8L784 Nitrosylation 1 Aconitase ARATH At2g05710 Chloroplast* Fragment 3 Aconitase ( )* ORYSA gi Mit. Matrix Carbonylation 4 Aconitate hydratase cytoplasmic ARATH At4g35830 Cytoplasm* Carbonylation 5 Aconitate hydratase cytoplasmic ARATH At2g05710 Chloroplast* Carbonylation 5 Actin 2/7 ARATH NP_ Nitrosylation 1 Actin 7 ARATH At5g09810 Cytoplasm* Carbonylation 5 Actin depolymerising factor 3-like ARATH NP_ Nitrosylation 1 Acyl carrier protein family ARATH NP_ Nitrosylation 1 Adenosine kinase 1 ARATH NP_ Nitrosylation 1 Adenosylhomocysteinase ARATH CAB09795 Nitrosylation 1 Alanine-glyoxylate aminotransferase ( )* ORYSA gi Peroxisome Carbonylation 4 Alcohol dehydrogenase ARATH At1g77120 Cytoplasm* Carbonylation 5 Aldehyde dehydrogenase ORYSA gi Mit. Trp-oxidation 2 Aldehyde dehydrogenase ( ) ORYSA gi Mit. Matrix Carbonylation 4 Aldose reductase ARATH At5g01670 Cytoplasm* Carbonylation 5 Aminomethyltransferase ARATH gi Nitrosylation 1 Aminomethyltransferase (glycine cleavage system T-protein) ARATH O65396 Mit. Carbonylation 6 Aminopeptidase ARATH At2g24200 Cytoplasm* Carbonylation 5 Annexin ARATH CAA67608 Nitrosylation 1 Ascorbate peroxidase ( )* ORYSA gi Unclear Carbonylation 4 Asp kinase/homoserine dehydrogenase ARATH At1g31230 Chloroplast Carbonylation 7 ATP synthase 27 kda subunit (ATP7) ARATH At2g21870 Mit.* Fragment 3 ATP synthase alpha subunit ARATH ATP1 Other* Fragment 3 ATP synthase beta subunit ARATH At5g08670 Mit.* Fragment 3 ATP synthase CF1 alpha chain ARATH NP_ Nitrosylation 1 ATP synthase CF1 beta chain ARATH NP_ Nitrosylation 1 ATP synthase α-subunit ( )* ORYSA gi Mit. Matrix Carbonylation 4 ATP synthase β-chain (mitochondrial precursor) ARATH At5g08670 Mit.* Carbonylation 5 ATP synthase β-subunit ( ) ORYSA gi Mit. Matrix Carbonylation 4 ATP synthase β-subunit, chloroplast ARATH gi Chloroplast Carbonylation 6 Basic chitinase ARATH A Nitrosylation 1 Calmodulin* ORYSA gi ER Carbonylation 4 Calreticulin 1 (Precursor) ARATH At1g56340 Secreted* Carbonylation 5 Carbonic anhydrase 2 ARATH P42737 Nitrosylation 1 Carbonic anhydrase, chloroplast precursor ARATH NP_ Nitrosylation 1 Catalase* RICCO Peroxisome Carbonylation 8 Catalase PEA Carbonylation 9 Catalase ( )* ORYSA gi Peroxisome Carbonylation 4 CBS domain containing protein ARATH NP_ Nitrosylation 1 Cell division cycle protein (CDC48) ARATH At3g09840 Cytoplasm* Carbonylation 5 Chaperonin (cpn 10) ARATH NP_ Nitrosylation 1 Chaperonin 10 ARATH gi Nitrosylation 1 Chaperonin 60 beta precursor ARATH JT0901 Nitrosylation 1 Chaperonin Cpn21, chloroplast ARATH NP_ Nitrosylation 1 Chaperonin hsp60 precursor ARATH gi Nitrosylation 1 Chitinase* ORYSA gi Vac./Apopl. Carbonylation 4 Chlorophyll a/b-binding protein ARATH Several hits Chloroplast Carbonylation 6 Citrate synthase ( ) ORYSA gi Mit. Matrix Carbonylation 4 Cold-regulated protein (cor15a) ARATH NP_ Nitrosylation 1 Cold-regulated protein (cor15b) ARATH NP_ Nitrosylation 1 Complex I 76 kda subunit (mitochondrial) SOLTU gi Mit. Trp-oxidation 2 Copper homeostasis factor ARATH NP_ Nitrosylation 1 β-cruciferin 12S (Seed storage protein fragment) ARATH At1g03880 Secreted* Carbonylation 5-1 -

2 β-cruciferin 12S (Seed storage protein fragment) ARATH At5g44120 Mit.* Carbonylation 5 β-cruciferin 12S (Seed storage protein fragment) ARATH At4g28520 Secreted* Carbonylation 5 Cu/Zn Superoxide dismutase ARATH NP_ Nitrosylation 1 Cupin family protein ARATH At1g03890 Secreted* Carbonylation 5 Cys synthase [O-acetylserine (thiol)lyase] ARATH At3g22460 Chloroplast Carbonylation 7 Cysteine proteinase inhibitor ARATH NP_ Nitrosylation 1 Cysteine synthase* ORYSA gi Mit. Matrix Carbonylation 4 Cytochrome b 5 reductase ( ) ORYSA gi Mit. OM M Carbonylation 4 Cytochrome c* ORYSA gi Mit. IMS Carbonylation 4 Cytochrome c oxidase subunit 2 ORYSA gi Mit. Trp-oxidation 2 Cytochrome P450 ARATH At4g15396 Secretory Carbonylation 7 Dihydrolipoamide dehydrogenase ( ) ORYSA gi Mit. Matrix Carbonylation 4 Dihydrolipoamide S-acetyltransferase ARATH At3g25860 Chloroplast* Carbonylation 5 Dihydrolipoamide S-acetyltransferase ( )* ORYSA gi Mit. Matrix Carbonylation 4 DNA topoisomerase I ARATH At5g55300 Cytoplasm* Carbonylation 5 DnaK-type molecular chaperone BiP ARATH At5g42020 Secreted* Carbonylation 5 DnaK-type molecular chaperone BiP ARATH gi Nitrosylation 1 Elongation factor 1B alpha subunit ARATH NP_ Nitrosylation 1 Elongation factor 1-γ 2 ARATH At1g57720 Cytoplasm* Carbonylation 5 Elongation factor 1-β-γ ARATH At1g09640 Cytoplasm* Carbonylation 5 Elongation factor EF-2 ARATH gi Nitrosylation 1 Elongation factor EF-2 ARATH At1g56070 Cytoplasm* Carbonylation 5 Elongation factor Tu ARATH NP_ Nitrosylation 1 Endomembrane-associated protein ARATH NP_ Nitrosylation 1 Endopeptidase Clp protease ATP-binding subunit ARATH NP_ Nitrosylation 1 Enolase ARATH At2g36530 Cytoplasm* Carbonylation 5 Enoyl-[acyl-carrier-protein] reductase ARATH At2g05990 Chloroplast* Carbonylation 5 Eukaryotic initiation factor 4A-1 ARATH At3g13920 Cytoplasm* Carbonylation 5 Expressed protein ARATH NP_ Nitrosylation 1 Expressed protein ARATH At1g05510 Cytoplasm* Carbonylation 5 Ferric leghemoglobin reductase ( )* ORYSA gi Mit. Matrix Carbonylation 4 Formate dehydrogenase ( ) ORYSA gi Mit. Matrix Carbonylation 4 Formate dehydrogenase, mitochondrial precursor (NAD-dependent formate dehydrogenase) (FDH) ORYSA gi Mit. Trp-oxidation 2 Fru-bisphosphate aldolase ARATH At2g36460 Cytoplasm* Carbonylation 5 Fructose 1,6-biphosphate aldolase, putative ARATH NP_ Nitrosylation 1 Fructose bisphosphate aldolase, putative ARATH AAK59548 Nitrosylation 1 Fructose-bisphosphate aldolase ( )* ORYSA gi Mit. Matrix Carbonylation 4 Fumarase ( )* ORYSA gi Mit. Matrix Carbonylation 4 Glutamate dehydrogenase ( ) ORYSA gi Mit. Matrix Carbonylation 4 Glutamate dehydrogenase 2 ARATH NP_ Nitrosylation 1 Glutamine synthase precursor, chloroplast ARATH NP_ Nitrosylation 1 Glutaredoxin, putative ARATH NP_ Nitrosylation 1 Glutathione peroxidase, putative ARATH NP_ Nitrosylation 1 Glutathione reductase PEA Carbonylation 9 Glutathione-S-transferase, putative ARATH NP_ Nitrosylation 1 Glyceraldehyde-3-phosphate dehydrogenase C subunit ARATH NP_ Nitrosylation 1 Glyceraldehyde-3-phosphate dehydrogenase (NADP) A precursor, chloroplast ARATH NP_ Nitrosylation 1 Glyceraldehyde-3-phosphate dehydrogenase (NADP) B precursor, chloroplast ARATH JQ1286 Nitrosylation 1 Glyceraldehyde-3-phosphate dehydrogenase cytosolic ARATH At3g04120 Cytoplasm* Carbonylation 5 Glyceraldehyde-3-phosphate dehydrogenase, cytosolic ARATH P25858 Nitrosylation 1 Glycine decarboxylase complex H protein precursor related ARATH NP_ Nitrosylation 1 Glycine decarboxylase H-protein* ORYSA gi Mit. Matrix Carbonylation 4 Glycine decarboxylase P-protein ( )* ORYSA gi Mit. Matrix Carbonylation 4 Glycine decarboxylase T protein ORYSA gi Mit. Matrix Carbonylation 4 Glycine dehydrogenase (glycine cleavage system P-protein) ARATH O80988 Mit. Carbonylation 6 Glycine dehydrogenase (putative) ORYSA gi Mit. Trp-oxidation 2 Glycine dehydrogenase P protein ORYSA gi Mit. Trp-oxidation 2-2 -

3 Glycine hydroxymethyltransferase-like protein ARATH gi Mit. Carbonylation 6 Glycine-rich RNA-binding protein 1A ARATH NP_ Nitrosylation 1 Glycine-rich RNA-binding protein 2 ARATH NP_ Nitrosylation 1 Glycine-rich RNA-binding protein 8 ARATH NP_ Nitrosylation 1 Glycolate oxidase ( )* ORYSA gi Peroxisome Carbonylation 4 Glycosyl hydrolase family 1 protein ARATH At3g09260 Secreted* Carbonylation 5 Glycosyl hydrolase family 1 protein ARATH At3g21370 Secreted* Carbonylation 5 Heat shock cognate 70 kd ARATH At3g09440 Cytoplasm* Carbonylation 5 Heat shock cognate 70 kd ARATH At5g02500 Cytoplasm* Carbonylation 5 Heat shock cognate 70-1 ARATH CAA54419 Nitrosylation 1 Heat shock protein 70.2 ARATH NP_ Nitrosylation 1 Heat shock protein 81 kda ARATH BAA00615 Nitrosylation 1 Heat shock protein 90, putative ARATH AAL49788 Nitrosylation 1 Hsp60 ORYSA gi Mit. Matrix Carbonylation 4 Hsp70 ORYSA gi Mit. Matrix Carbonylation 4 Hsp70 ARATH Several hits Chloroplast Carbonylation 6 Hsp70 ARATH At3g12580 Cytoplasm* Carbonylation 5 Hsp70 ARATH At4g24280 Chloroplast* Carbonylation 5 Hsp70 ARATH At5g09590 Mit.* Carbonylation 5 Hsp70 ARATH At1g16030 Cytoplasm* Carbonylation 5 Hydrolase α/β-fold family protein ARATH At3g54240 Secreted* Carbonylation 5 (S)-2-Hydroxy-acid oxidase ( ) ORYSA gi Peroxisome Carbonylation 4 Indole-3-glycerol phosphate synthase ARATH At2g04400 Chloroplast* Carbonylation 5 Initiation factor 5A-4-related ARATH NP_ Nitrosylation 1 Initiation factor eif-4a1 ARATH CAC43286 Nitrosylation 1 Isocitrate dehydrogenase ARATH At1g65930 Cytoplasm* Carbonylation 5 Isocitrate lyase* RICCO Peroxisome Carbonylation 8 3-Isopropylmalate dehydrogenase like protein* ORYSA gi Mit. Matrix Carbonylation 4 Jasmonate inducible protein (myrosinase binding protein) ARATH NP_ Nitrosylation 1 Jasmonate inducible protein-like ARATH At2g33070 Cytoplasm* Carbonylation 5 LEA ARATH At2g42560 Cytoplasm* Carbonylation 5 Low-temperature-induced 65-kD protein ARATH At5g52300 Cytoplasm* Carbonylation 5 Malate dehydrogenase ( ) ORYSA gi Mit. Matrix Carbonylation 4 Malate dehydrogenase cytoplasmic 1 ARATH At1g04410 Cytoplasm* Carbonylation 5 Malate dehydrogenase, cytosolic, putative ARATH NP_ Nitrosylation 1 Malate dehydrogenase, mitochondrial ARATH AAM64855 Nitrosylation 1 Malate dehydrogenase, mitochondrial, putative ARATH NP_ Nitrosylation 1 Malate oxidoreductase or malic enzyme ARATH At2g19900 Cytoplasm* Carbonylation 5 Malate synthase* RICCO Peroxisome Carbonylation 8 Manganese superoxide dismutase PEA Carbonylation 9 4-Methyl-5(b-hydroxyethyl)-thiazole monophosphate biosynthesis protein, putative ARATH NP_ Nitrosylation 1 5-Methyl-tetrahydropteroyl-tri-Glu-homocysteine methyltransferase ARATH At5g17920 Cytoplasm* Carbonylation 5 5-Methyl-tetrahydropteroyltriglutamate-homocysteine S- methyltransferase (Methionine synthase) ARATH NP_ Nitrosylation 1 Methylmalonate-semialdehyde dehydrogenase ORYSA gi Mit. Trp-oxidation 2 Methylmalonate-semialdehyde dehydrogenase ( ) ORYSA gi Mit. Matrix Carbonylation 4 Mitochondrial processing peptidase, α-subunit SOLTU gi Mit. Trp-oxidation 2 Mitochondrial processing peptidase, α-subunit SOLTU gi Mit. Trp-oxidation 2 Mitochondrial processing peptidase, β-subunit SOLTU gi Mit. Trp-oxidation 2 Mitochondroial F1 ATP synthase β-subunit ARATH gi Mit. Carbonylation 6 NAD malate dehydrogenase ARATH At1g53240 Mit.* Fragment 3 NAD + -isocitrate dehydrogenase ( ) ORYSA gi Mit. Matrix Carbonylation 4 NAD + -malic enzyme 59 kda iso form ( )* ORYSA gi Mit. Matrix Carbonylation 4 NADH2 dehydrogenase (ubiquinone) (EC ) chain 7 SOLTU gi Mit. Trp-oxidation 2 Nonspecific lipid transfer protein 2 ARATH NP_ Nitrosylation 1 Nucleoside diphosphate kinase ARATH S31446 Nitrosylation 1 Nucleoside diphosphate kinase III ARATH At4g11010 Chloroplast* Fragment 3-3 -

4 O-acetylserine (thiol) lyase (Cysteine synthase) ARATH CAA58893 Nitrosylation 1 Oxalate oxidase ( ) ORYSA gi Apoplast Carbonylation 4 3-Oxoacyl-[acylcarrier-protein] synthase 1 ARATH At5g46290 Chloroplast* Carbonylation 5 Oxygen evolving complex, 33 kda subunit ARATH gi Chloroplast Carbonylation 6 Peptidase M1 family protein ARATH At1g63770 Chloroplast* Carbonylation 5 Peptidylprolyl isomerase ARATH NP_ Nitrosylation 1 Peptidylprolyl isomerase ( ) / cyclophilin* ORYSA gi Mit./Cyt./Mic. Carbonylation 4 Peptidylprolyl isomerase ROC4 ARATH NP_ Nitrosylation 1 Peroxiredoxin - like protein ARATH NP_ Nitrosylation 1 Peroxiredoxin (/unnamed protein product)* ORYSA gi Mit. Matrix Carbonylation 4 Peroxiredoxin-related ARATH AAF66133 Nitrosylation 1 2-Phosphoglycerate hydrolase (enolase) ARATH NP_ Nitrosylation 1 3-Phosphoglycerate dehydrogenase, putative ARATH NP_ Nitrosylation 1 Phosphoglycerate kinase ARATH NP_ Nitrosylation 1 Phosphoglycerate kinase related ARATH NP_ Nitrosylation 1 Photosystem I subunit II precursor ARATH CAB52677 Nitrosylation 1 Photosystem II D2 protein, fragment ARATH AAO13251 Nitrosylation 1 Photosystem II oxygen-evolving complex 23 ARATH NP_ Nitrosylation 1 Photosystem II oxygen-evolving complex 33 ARATH NP_ Nitrosylation 1 Photosystem II P kda protein ARATH NP_ Nitrosylation 1 P-protein-like protein ARATH gi Mit. Carbonylation 6 Probable major latex protein ARATH AAK63869 Nitrosylation 1 Probable malate dehydrogenase precursor, glyoxysomal ARATH NP_ Nitrosylation 1 Probable photosystem II stability protein ARATH AAK74049 Nitrosylation 1 Probable superoxide dismutase (EC ) (Mn) 2 precursor ORYSA gi Mit. Trp-oxidation 2 Probable triosephosphate isomerase ARATH AAF70259 Nitrosylation 1 Prolyl trna synthetase ( )* ORYSA gi Chloroplast Carbonylation 4 Protein disulfide isomerase ARATH At1g21750 Secreted* Carbonylation 5 Putative alanine aminotransferase ARATH AAK25905 Nitrosylation 1 Putative aminotransferase* ORYSA gi Chloroplast Carbonylation 4 Putative cation/h + exchanger ARATH At5g58460 Membrane Carbonylation 7 Putative elongation factor 1B gamma ARATH AAK59587 Nitrosylation 1 Putative mitochondrial NADH:ubiquinone oxidoreductase 29 kda subunit ORYSA gi Mit. Trp-oxidation 2 Putative mitochondrial processing peptidase, β ORYSA gi Mit. Trp-oxidation 2 Putative monodehydroascorbate reductase ORYSA gi Mit. Trp-oxidation 2 Putative NADPH:quinone oxidoreductase* ORYSA gi Mit. Matrix Carbonylation 4 Putative RNA-binding protein ARATH Q8LAU2 Nitrosylation 1 Putative transketolase* ORYSA gi Chloroplast Carbonylation 4 Putative transposase ARATH At2g14140 Cytosol/nuclear Carbonylation 7 Pyruvate dehydrogenase E2 subunit ARATH At3g52200 Mit.* Fragment 3 Pyruvate dehydrogenase, E1α-subunit ( )* ORYSA gi Mit. Matrix Carbonylation 4 Pyruvate dehydrogenase, E1β-subunit ( ) ORYSA gi Mit. Matrix Carbonylation 4 Pyruvate-orthophosphate dikinase ARATH At4g15530 Cytoplasm* Carbonylation 5 Riblose 1,5-bisphosphate carboxylase/oxygenase large chain ARATH NP_ Nitrosylation 1 Ribonuclease ARATH NP_ Nitrosylation 1 Ribosomal protein 50S ARATH AAM64725 Nitrosylation 1 Ribosomal protein L12 ARATH NP_ Nitrosylation 1 Ribosomal protein S14 (40S) ARATH NP_ Nitrosylation 1 Ribosomal protein S2* ORYSA gi Chloroplast Carbonylation 4 Ribulose bisphosphate carboxylase small chain 1a precursor ARATH P10795 Nitrosylation 1 Ribulose bisphosphate carboxylase small chain 1b precursor ARATH NP_ Nitrosylation 1 Ribulose-bisphosphate carboxylase small chain B2 precursor ARATH P10797 Nitrosylation 1 Rieske Fe-S protein ARATH CAC03598 Nitrosylation 1 RNA-binding protein-like ARATH NP_ Nitrosylation 1 Rubisco PEA Carbonylation 9 Rubisco activase, large subunit ARATH AAA20202 Nitrosylation 1 Rubisco large chain ARATH AtCg00490 Chloroplast* Carbonylation 5 Rubisco large subunit ARATH AtCg00490 Chloroplast Carbonylation 7 RuBisCO small subunit A ( )* ORYSA gi Chloroplast Carbonylation 4 Rubisco subunit binding-protein β-subunit ORYSA gi Chloroplast Carbonylation 4-4 -

5 Rubisco-activase splice form 1 ARATH gi Chloroplast Carbonylation 6 Rubisco-activase splice form 2 ARATH gi Chloroplast Carbonylation 6 Rubisco-large subunit ARATH gi Chloroplast Carbonylation 6 S-Adenosylmethionine synthetase, putative ARATH AAO11581 Nitrosylation 1 Serine hydroxymethyltransferase ( )* ORYSA gi Mit. Matrix Carbonylation 4 SET domain transcriptional regulatory family ARATH Q8VY03 Nitrosylation 1 Storage proteins 7S ARATH At3g22640 Secreted* Carbonylation 5 Succinyl CoA ligase alpha subunit ARATH At5g08300 Chloroplast* Fragment 3 Succinyl CoA ligase beta subunit ARATH At2g20420 Mit.* Fragment 3 Succinyl-CoA-ligase, α-subunit ( )* ORYSA gi Mit. Matrix Carbonylation 4 Succinyl-CoA-ligase, β-subunit ( )* ORYSA gi Mit. Matrix Carbonylation 4 Superoxide dismutase (Mn) (EC ) ORYSA gi Mit. Matrix Carbonylation 4 Thioredoxin f1 ARATH AAD35003 Nitrosylation 1 Thiosulfate sulfurtransferase* ORYSA gi Mit. Matrix Carbonylation 4 Thylakoid lumenal 16.5 kda protein, chloroplast precursor ARATH O22773 Nitrosylation 1 Transcription activator ARATH At3g52910 Cytosol/nuclear Carbonylation 7 Transitional endoplasmic reticulum ATPase ARATH T48355 Nitrosylation 1 Transketolase ARATH At3g60750 Chloroplast* Carbonylation 5 Transketolase-like protein ARATH gi Nitrosylation 1 Translation elongation factor eef-1alpha chain ARATH gi Nitrosylation 1 Translational elongation factor TuM ORYSA gi Mit. Matrix Carbonylation 4 Translationally controlled tumor protein-like protein ARATH Q8L973 Nitrosylation 1 Trigger factor-related ARATH NP_ Nitrosylation 1 Triosephosphate isomerase ARATH gi Nitrosylation 1 Tubulin alpha 6 chain ARATH P29511 Nitrosylation 1 Tubulin beta 4 chain ARATH P24636 Nitrosylation 1 Type 2 peroxiredoxin-related ARATH NP_ Nitrosylation 1 UDP-Glc pyrophosphorylase ARATH At5g17310 Secreted* Carbonylation 5 Unknown protein ARATH AAK59490 Nitrosylation 1 Unnamed protein product* ORYSA gi Chloroplast Carbonylation 4 Unnamed protein product (superoxide dismutase) SOLTU gi Mit. Trp-oxidation 2 Voltage-dependent anion channel ORYSA gi Mit. OM M Carbonylation 4 Voltage-dependent anion channel (porin) ARATH At5g15090 Other* Fragment 3 Zinc-finger, C3HC4 (RING finger) ARATH At5g60710 Nuclear Carbonylation 7 a) Protein names are given as in original references. Minor rearrangements of names have been done in order to obtain proteins in alphabetic order. Proteins marked with an asterisk were only identified as oxidized after in vitro oxidation. b) Indicating the organisms used in respective original study: ARATH, Arabidopsis thaliana; ORYSA, Oryza sativa; PEA, Pisum sativum; RICCO, Ricinus communis; SOLTU, Solanum tuberosum. c) When accession no. or other identifiers was included in the original publication, these are included in the table. In cases where more than one identifier was given, the first one has been included. In cases where the original entry has been discontinued the new entries are indicated. d) Localization of the protein as specified in the original publication, or * based on TargetP. Apopl., apoplast; Cyt., cytosol; ER, endoplasmatic reticulum; IMS, intermembrane space; OMM, outer mitochondrial membrane; Mic., microbodies; Mit., mitochondria; Vac., vacuole. e) Type of protein modification identified. f) Reference: 1. Lindermayr C, Saalbach G, Durner J Proteomic identification of S-nitrosylated proteins in Arabidopsis. Plant Physiol. 137: Møller IM, Kristensen BK Protein oxidation in plant mitochondria detected as oxidized tryptophan. Free Radicals Biol. Med. 40: Sweetlove LJ, Heazlewood JL, Herald V, Holtzapffel R, Day DA, Leaver CJ, Millar AH The impact of oxidative stress on Arabidopsis mitochondria. Plant Journal 32: Kristensen BK, Askerlund P, Bykova NV, Egsgaard H, Møller IM Identification of oxidised proteins in the matrix of rice leaf mitochondria by immunoprecipitation and two-dimensional liquid chromatography-tandem mass spectrometry. Phytochemistry 65: Job C, Rajjou L, Lovigny, Y, Belghazi M, Job D Patterns of protein oxidation in Arabidopsis seeds and during germination. Plant Physiol. 138:

6 6. Johansson E, Olsson O, Nyström T Progression and specificity of protein oxidation in the life cycle of Arabidopsis thaliana. J. Biol. Chem. 279: Davletova S, Rizhsky L, Liang HJ, Shengqiang Z, Oliver DJ et al Cytosolic ascorbate peroxidase 1 is a central component of the reactive oxygen gene network of Arabidopsis. Plant Cell 17: Nguyen AT, Donaldson RP Metal-catalyzed oxidation induces carbonylation of peroxisomal proteins and loss of enzymatic activities. Arch. Biochem. Biophys. 439: Romero-Puertas MC, Palma JM, Gómez M, del Rio LA, Sandalio LM Cadmium causes the oxidative modification of proteins in pea plants. Plant Cell Environ. 25: