Supplemental Table 1. List of identified oxidatively modified plant proteins Protein (EC number) a) Species b) Acc. No. / ID c) Localization d) Modification e) Ref. f) 14-3-3 protein GF14 ARATH NP_564167 Nitrosylation 1 Acidic ribosomal protein P2 (60S) ARATH NP_180340 Nitrosylation 1 Aconitase ORYSA gi 29027432 Mit. Trp-oxidation 2 Aconitase ARATH Q8L784 Nitrosylation 1 Aconitase ARATH At2g05710 Chloroplast* Fragment 3 Aconitase (4.2.1.3)* ORYSA gi 30678219 Mit. Matrix Carbonylation 4 Aconitate hydratase cytoplasmic ARATH At4g35830 Cytoplasm* Carbonylation 5 Aconitate hydratase cytoplasmic ARATH At2g05710 Chloroplast* Carbonylation 5 Actin 2/7 ARATH NP_196543 Nitrosylation 1 Actin 7 ARATH At5g09810 Cytoplasm* Carbonylation 5 Actin depolymerising factor 3-like ARATH NP_851227 Nitrosylation 1 Acyl carrier protein family ARATH NP_194235 Nitrosylation 1 Adenosine kinase 1 ARATH NP_187593 Nitrosylation 1 Adenosylhomocysteinase ARATH CAB09795 Nitrosylation 1 Alanine-glyoxylate aminotransferase (2.6.1.44)* ORYSA gi 15225026 Peroxisome Carbonylation 4 Alcohol dehydrogenase ARATH At1g77120 Cytoplasm* Carbonylation 5 Aldehyde dehydrogenase ORYSA gi 8163730 Mit. Trp-oxidation 2 Aldehyde dehydrogenase (1.2.1.3) ORYSA gi 11691926 Mit. Matrix Carbonylation 4 Aldose reductase ARATH At5g01670 Cytoplasm* Carbonylation 5 Aminomethyltransferase ARATH gi 18206365 Nitrosylation 1 Aminomethyltransferase (glycine cleavage system T-protein) ARATH O65396 Mit. Carbonylation 6 Aminopeptidase ARATH At2g24200 Cytoplasm* Carbonylation 5 Annexin ARATH CAA67608 Nitrosylation 1 Ascorbate peroxidase (1.11.1.11)* ORYSA gi 15808779 Unclear Carbonylation 4 Asp kinase/homoserine dehydrogenase ARATH At1g31230 Chloroplast Carbonylation 7 ATP synthase 27 kda subunit (ATP7) ARATH At2g21870 Mit.* Fragment 3 ATP synthase alpha subunit ARATH ATP1 Other* Fragment 3 ATP synthase beta subunit ARATH At5g08670 Mit.* Fragment 3 ATP synthase CF1 alpha chain ARATH NP_051044 Nitrosylation 1 ATP synthase CF1 beta chain ARATH NP_051066 Nitrosylation 1 ATP synthase α-subunit (3.6.3.14)* ORYSA gi 9408184 Mit. Matrix Carbonylation 4 ATP synthase β-chain (mitochondrial precursor) ARATH At5g08670 Mit.* Carbonylation 5 ATP synthase β-subunit (3.6.3.14) ORYSA gi 84028177 Mit. Matrix Carbonylation 4 ATP synthase β-subunit, chloroplast ARATH gi 7525040 Chloroplast Carbonylation 6 Basic chitinase ARATH 1710349A Nitrosylation 1 Calmodulin* ORYSA gi 16225 ER Carbonylation 4 Calreticulin 1 (Precursor) ARATH At1g56340 Secreted* Carbonylation 5 Carbonic anhydrase 2 ARATH P42737 Nitrosylation 1 Carbonic anhydrase, chloroplast precursor ARATH NP_850490 Nitrosylation 1 Catalase* RICCO Peroxisome Carbonylation 8 Catalase PEA Carbonylation 9 Catalase (1.11.1.6)* ORYSA gi 20192 Peroxisome Carbonylation 4 CBS domain containing protein ARATH NP_196647 Nitrosylation 1 Cell division cycle protein (CDC48) ARATH At3g09840 Cytoplasm* Carbonylation 5 Chaperonin (cpn 10) ARATH NP_563961 Nitrosylation 1 Chaperonin 10 ARATH gi 12643263 Nitrosylation 1 Chaperonin 60 beta precursor ARATH JT0901 Nitrosylation 1 Chaperonin Cpn21, chloroplast ARATH NP_197572 Nitrosylation 1 Chaperonin hsp60 precursor ARATH gi 12644189 Nitrosylation 1 Chitinase* ORYSA gi 18146827 Vac./Apopl. Carbonylation 4 Chlorophyll a/b-binding protein ARATH Several hits Chloroplast Carbonylation 6 Citrate synthase (2.3.3.1) ORYSA gi 11066954 Mit. Matrix Carbonylation 4 Cold-regulated protein (cor15a) ARATH NP_181782 Nitrosylation 1 Cold-regulated protein (cor15b) ARATH NP_181781 Nitrosylation 1 Complex I 76 kda subunit (mitochondrial) SOLTU gi 758340 Mit. Trp-oxidation 2 Copper homeostasis factor ARATH NP_564870 Nitrosylation 1 β-cruciferin 12S (Seed storage protein fragment) ARATH At1g03880 Secreted* Carbonylation 5-1 -
β-cruciferin 12S (Seed storage protein fragment) ARATH At5g44120 Mit.* Carbonylation 5 β-cruciferin 12S (Seed storage protein fragment) ARATH At4g28520 Secreted* Carbonylation 5 Cu/Zn Superoxide dismutase ARATH NP_172360 Nitrosylation 1 Cupin family protein ARATH At1g03890 Secreted* Carbonylation 5 Cys synthase [O-acetylserine (thiol)lyase] ARATH At3g22460 Chloroplast Carbonylation 7 Cysteine proteinase inhibitor ARATH NP_193383 Nitrosylation 1 Cysteine synthase* ORYSA gi 90265107 Mit. Matrix Carbonylation 4 Cytochrome b 5 reductase (1.6.2.2) ORYSA gi 15128231 Mit. OM M Carbonylation 4 Cytochrome c* ORYSA gi 2394300 Mit. IMS Carbonylation 4 Cytochrome c oxidase subunit 2 ORYSA gi 40795155 Mit. Trp-oxidation 2 Cytochrome P450 ARATH At4g15396 Secretory Carbonylation 7 Dihydrolipoamide dehydrogenase (1.8.1.4) ORYSA gi 13365781 Mit. Matrix Carbonylation 4 Dihydrolipoamide S-acetyltransferase ARATH At3g25860 Chloroplast* Carbonylation 5 Dihydrolipoamide S-acetyltransferase (2.3.1.12)* ORYSA gi 5669871 Mit. Matrix Carbonylation 4 DNA topoisomerase I ARATH At5g55300 Cytoplasm* Carbonylation 5 DnaK-type molecular chaperone BiP ARATH At5g42020 Secreted* Carbonylation 5 DnaK-type molecular chaperone BiP ARATH gi 12643245 Nitrosylation 1 Elongation factor 1B alpha subunit ARATH NP_568375 Nitrosylation 1 Elongation factor 1-γ 2 ARATH At1g57720 Cytoplasm* Carbonylation 5 Elongation factor 1-β-γ ARATH At1g09640 Cytoplasm* Carbonylation 5 Elongation factor EF-2 ARATH gi 75337209 Nitrosylation 1 Elongation factor EF-2 ARATH At1g56070 Cytoplasm* Carbonylation 5 Elongation factor Tu ARATH NP_193769 Nitrosylation 1 Endomembrane-associated protein ARATH NP_193759 Nitrosylation 1 Endopeptidase Clp protease ATP-binding subunit ARATH NP_566912 Nitrosylation 1 Enolase ARATH At2g36530 Cytoplasm* Carbonylation 5 Enoyl-[acyl-carrier-protein] reductase ARATH At2g05990 Chloroplast* Carbonylation 5 Eukaryotic initiation factor 4A-1 ARATH At3g13920 Cytoplasm* Carbonylation 5 Expressed protein ARATH NP_564691 Nitrosylation 1 Expressed protein ARATH At1g05510 Cytoplasm* Carbonylation 5 Ferric leghemoglobin reductase (1.6.2.6)* ORYSA gi 5823556 Mit. Matrix Carbonylation 4 Formate dehydrogenase (1.2.1.2) ORYSA gi 109909540 Mit. Matrix Carbonylation 4 Formate dehydrogenase, mitochondrial precursor (NAD-dependent formate dehydrogenase) (FDH) ORYSA gi 109909540 Mit. Trp-oxidation 2 Fru-bisphosphate aldolase ARATH At2g36460 Cytoplasm* Carbonylation 5 Fructose 1,6-biphosphate aldolase, putative ARATH NP_190861 Nitrosylation 1 Fructose bisphosphate aldolase, putative ARATH AAK59548 Nitrosylation 1 Fructose-bisphosphate aldolase (4.1.2.13)* ORYSA gi 14090214 Mit. Matrix Carbonylation 4 Fumarase (4.2.1.2)* ORYSA gi 75319718 Mit. Matrix Carbonylation 4 Glutamate dehydrogenase (1.4.1.2) ORYSA gi 12643763 Mit. Matrix Carbonylation 4 Glutamate dehydrogenase 2 ARATH NP_196361 Nitrosylation 1 Glutamine synthase precursor, chloroplast ARATH NP_198413 Nitrosylation 1 Glutaredoxin, putative ARATH NP_198853 Nitrosylation 1 Glutathione peroxidase, putative ARATH NP_192897 Nitrosylation 1 Glutathione reductase PEA Carbonylation 9 Glutathione-S-transferase, putative ARATH NP_565178 Nitrosylation 1 Glyceraldehyde-3-phosphate dehydrogenase C subunit ARATH NP_187062 Nitrosylation 1 Glyceraldehyde-3-phosphate dehydrogenase (NADP) A precursor, chloroplast ARATH NP_566796 Nitrosylation 1 Glyceraldehyde-3-phosphate dehydrogenase (NADP) B precursor, chloroplast ARATH JQ1286 Nitrosylation 1 Glyceraldehyde-3-phosphate dehydrogenase cytosolic ARATH At3g04120 Cytoplasm* Carbonylation 5 Glyceraldehyde-3-phosphate dehydrogenase, cytosolic ARATH P25858 Nitrosylation 1 Glycine decarboxylase complex H protein precursor related ARATH NP_181080 Nitrosylation 1 Glycine decarboxylase H-protein* ORYSA gi 13786469 Mit. Matrix Carbonylation 4 Glycine decarboxylase P-protein (1.4.4.2)* ORYSA gi 13366201 Mit. Matrix Carbonylation 4 Glycine decarboxylase T protein ORYSA gi 1346121 Mit. Matrix Carbonylation 4 Glycine dehydrogenase (glycine cleavage system P-protein) ARATH O80988 Mit. Carbonylation 6 Glycine dehydrogenase (putative) ORYSA gi 51090904 Mit. Trp-oxidation 2 Glycine dehydrogenase P protein ORYSA gi 33641855 Mit. Trp-oxidation 2-2 -
Glycine hydroxymethyltransferase-like protein ARATH gi 15235745 Mit. Carbonylation 6 Glycine-rich RNA-binding protein 1A ARATH NP_179760 Nitrosylation 1 Glycine-rich RNA-binding protein 2 ARATH NP_193121 Nitrosylation 1 Glycine-rich RNA-binding protein 8 ARATH NP_195637 Nitrosylation 1 Glycolate oxidase (1.1.3.15)* ORYSA gi 15231850 Peroxisome Carbonylation 4 Glycosyl hydrolase family 1 protein ARATH At3g09260 Secreted* Carbonylation 5 Glycosyl hydrolase family 1 protein ARATH At3g21370 Secreted* Carbonylation 5 Heat shock cognate 70 kd ARATH At3g09440 Cytoplasm* Carbonylation 5 Heat shock cognate 70 kd ARATH At5g02500 Cytoplasm* Carbonylation 5 Heat shock cognate 70-1 ARATH CAA54419 Nitrosylation 1 Heat shock protein 70.2 ARATH NP_195869 Nitrosylation 1 Heat shock protein 81 kda ARATH BAA00615 Nitrosylation 1 Heat shock protein 90, putative ARATH AAL49788 Nitrosylation 1 Hsp60 ORYSA gi 2506275 Mit. Matrix Carbonylation 4 Hsp70 ORYSA gi 399940 Mit. Matrix Carbonylation 4 Hsp70 ARATH Several hits Chloroplast Carbonylation 6 Hsp70 ARATH At3g12580 Cytoplasm* Carbonylation 5 Hsp70 ARATH At4g24280 Chloroplast* Carbonylation 5 Hsp70 ARATH At5g09590 Mit.* Carbonylation 5 Hsp70 ARATH At1g16030 Cytoplasm* Carbonylation 5 Hydrolase α/β-fold family protein ARATH At3g54240 Secreted* Carbonylation 5 (S)-2-Hydroxy-acid oxidase (1.1.3.15) ORYSA gi 75318006 Peroxisome Carbonylation 4 Indole-3-glycerol phosphate synthase ARATH At2g04400 Chloroplast* Carbonylation 5 Initiation factor 5A-4-related ARATH NP_173985 Nitrosylation 1 Initiation factor eif-4a1 ARATH CAC43286 Nitrosylation 1 Isocitrate dehydrogenase ARATH At1g65930 Cytoplasm* Carbonylation 5 Isocitrate lyase* RICCO Peroxisome Carbonylation 8 3-Isopropylmalate dehydrogenase like protein* ORYSA gi 75181264 Mit. Matrix Carbonylation 4 Jasmonate inducible protein (myrosinase binding protein) ARATH NP_188263 Nitrosylation 1 Jasmonate inducible protein-like ARATH At2g33070 Cytoplasm* Carbonylation 5 LEA ARATH At2g42560 Cytoplasm* Carbonylation 5 Low-temperature-induced 65-kD protein ARATH At5g52300 Cytoplasm* Carbonylation 5 Malate dehydrogenase (1.1.1.137) ORYSA gi 14164433 Mit. Matrix Carbonylation 4 Malate dehydrogenase cytoplasmic 1 ARATH At1g04410 Cytoplasm* Carbonylation 5 Malate dehydrogenase, cytosolic, putative ARATH NP_171936 Nitrosylation 1 Malate dehydrogenase, mitochondrial ARATH AAM64855 Nitrosylation 1 Malate dehydrogenase, mitochondrial, putative ARATH NP_188120 Nitrosylation 1 Malate oxidoreductase or malic enzyme ARATH At2g19900 Cytoplasm* Carbonylation 5 Malate synthase* RICCO Peroxisome Carbonylation 8 Manganese superoxide dismutase PEA Carbonylation 9 4-Methyl-5(b-hydroxyethyl)-thiazole monophosphate biosynthesis protein, putative ARATH NP_188117 Nitrosylation 1 5-Methyl-tetrahydropteroyl-tri-Glu-homocysteine methyltransferase ARATH At5g17920 Cytoplasm* Carbonylation 5 5-Methyl-tetrahydropteroyltriglutamate-homocysteine S- methyltransferase (Methionine synthase) ARATH NP_197294 Nitrosylation 1 Methylmalonate-semialdehyde dehydrogenase ORYSA gi 2895866 Mit. Trp-oxidation 2 Methylmalonate-semialdehyde dehydrogenase (1.2.1.27) ORYSA gi 75219570 Mit. Matrix Carbonylation 4 Mitochondrial processing peptidase, α-subunit SOLTU gi 587566 Mit. Trp-oxidation 2 Mitochondrial processing peptidase, α-subunit SOLTU gi 587564 Mit. Trp-oxidation 2 Mitochondrial processing peptidase, β-subunit SOLTU gi 21493 Mit. Trp-oxidation 2 Mitochondroial F1 ATP synthase β-subunit ARATH gi 17939849 Mit. Carbonylation 6 NAD malate dehydrogenase ARATH At1g53240 Mit.* Fragment 3 NAD + -isocitrate dehydrogenase (1.1.1.41) ORYSA gi 15237075 Mit. Matrix Carbonylation 4 NAD + -malic enzyme 59 kda iso form (1.1.1.39)* ORYSA gi 585452 Mit. Matrix Carbonylation 4 NADH2 dehydrogenase (ubiquinone) (EC 1.6.5.3) chain 7 SOLTU gi 75281479 Mit. Trp-oxidation 2 Nonspecific lipid transfer protein 2 ARATH NP_181387 Nitrosylation 1 Nucleoside diphosphate kinase ARATH S31446 Nitrosylation 1 Nucleoside diphosphate kinase III ARATH At4g11010 Chloroplast* Fragment 3-3 -
O-acetylserine (thiol) lyase (Cysteine synthase) ARATH CAA58893 Nitrosylation 1 Oxalate oxidase (1.2.3.4) ORYSA gi 6996619 Apoplast Carbonylation 4 3-Oxoacyl-[acylcarrier-protein] synthase 1 ARATH At5g46290 Chloroplast* Carbonylation 5 Oxygen evolving complex, 33 kda subunit ARATH gi 15230324 Chloroplast Carbonylation 6 Peptidase M1 family protein ARATH At1g63770 Chloroplast* Carbonylation 5 Peptidylprolyl isomerase ARATH NP_195213 Nitrosylation 1 Peptidylprolyl isomerase (5.2.1.8) / cyclophilin* ORYSA gi 75282067 Mit./Cyt./Mic. Carbonylation 4 Peptidylprolyl isomerase ROC4 ARATH NP_191762 Nitrosylation 1 Peroxiredoxin - like protein ARATH NP_190864 Nitrosylation 1 Peroxiredoxin (/unnamed protein product)* ORYSA gi 6630684 Mit. Matrix Carbonylation 4 Peroxiredoxin-related ARATH AAF66133 Nitrosylation 1 2-Phosphoglycerate hydrolase (enolase) ARATH NP_181192 Nitrosylation 1 3-Phosphoglycerate dehydrogenase, putative ARATH NP_195146 Nitrosylation 1 Phosphoglycerate kinase ARATH NP_178073 Nitrosylation 1 Phosphoglycerate kinase related ARATH NP_187884 Nitrosylation 1 Photosystem I subunit II precursor ARATH CAB52677 Nitrosylation 1 Photosystem II D2 protein, fragment ARATH AAO13251 Nitrosylation 1 Photosystem II oxygen-evolving complex 23 ARATH NP_172153 Nitrosylation 1 Photosystem II oxygen-evolving complex 33 ARATH NP_190651 Nitrosylation 1 Photosystem II P680 47 kda protein ARATH NP_051084 Nitrosylation 1 P-protein-like protein ARATH gi 14596025 Mit. Carbonylation 6 Probable major latex protein ARATH AAK63869 Nitrosylation 1 Probable malate dehydrogenase precursor, glyoxysomal ARATH NP_196528 Nitrosylation 1 Probable photosystem II stability protein ARATH AAK74049 Nitrosylation 1 Probable superoxide dismutase (EC 1.15.1.1) (Mn) 2 precursor ORYSA gi 75221505 Mit. Trp-oxidation 2 Probable triosephosphate isomerase ARATH AAF70259 Nitrosylation 1 Prolyl trna synthetase (6.1.1.15)* ORYSA gi 15237183 Chloroplast Carbonylation 4 Protein disulfide isomerase ARATH At1g21750 Secreted* Carbonylation 5 Putative alanine aminotransferase ARATH AAK25905 Nitrosylation 1 Putative aminotransferase* ORYSA gi 38344250 Chloroplast Carbonylation 4 Putative cation/h + exchanger ARATH At5g58460 Membrane Carbonylation 7 Putative elongation factor 1B gamma ARATH AAK59587 Nitrosylation 1 Putative mitochondrial NADH:ubiquinone oxidoreductase 29 kda subunit ORYSA gi 50908833 Mit. Trp-oxidation 2 Putative mitochondrial processing peptidase, β ORYSA gi 55771316 Mit. Trp-oxidation 2 Putative monodehydroascorbate reductase ORYSA gi 50941197 Mit. Trp-oxidation 2 Putative NADPH:quinone oxidoreductase* ORYSA gi 15528813 Mit. Matrix Carbonylation 4 Putative RNA-binding protein ARATH Q8LAU2 Nitrosylation 1 Putative transketolase* ORYSA gi 11875175 Chloroplast Carbonylation 4 Putative transposase ARATH At2g14140 Cytosol/nuclear Carbonylation 7 Pyruvate dehydrogenase E2 subunit ARATH At3g52200 Mit.* Fragment 3 Pyruvate dehydrogenase, E1α-subunit (1.2.4.1)* ORYSA gi 3851005 Mit. Matrix Carbonylation 4 Pyruvate dehydrogenase, E1β-subunit (1.2.4.1) ORYSA gi 3850999 Mit. Matrix Carbonylation 4 Pyruvate-orthophosphate dikinase ARATH At4g15530 Cytoplasm* Carbonylation 5 Riblose 1,5-bisphosphate carboxylase/oxygenase large chain ARATH NP_051067 Nitrosylation 1 Ribonuclease ARATH NP_178399 Nitrosylation 1 Ribosomal protein 50S ARATH AAM64725 Nitrosylation 1 Ribosomal protein L12 ARATH NP_181256 Nitrosylation 1 Ribosomal protein S14 (40S) ARATH NP_181158 Nitrosylation 1 Ribosomal protein S2* ORYSA gi 11466379 Chloroplast Carbonylation 4 Ribulose bisphosphate carboxylase small chain 1a precursor ARATH P10795 Nitrosylation 1 Ribulose bisphosphate carboxylase small chain 1b precursor ARATH NP_198659 Nitrosylation 1 Ribulose-bisphosphate carboxylase small chain B2 precursor ARATH P10797 Nitrosylation 1 Rieske Fe-S protein ARATH CAC03598 Nitrosylation 1 RNA-binding protein-like ARATH NP_188660 Nitrosylation 1 Rubisco PEA Carbonylation 9 Rubisco activase, large subunit ARATH AAA20202 Nitrosylation 1 Rubisco large chain ARATH AtCg00490 Chloroplast* Carbonylation 5 Rubisco large subunit ARATH AtCg00490 Chloroplast Carbonylation 7 RuBisCO small subunit A (4.1.1.39)* ORYSA gi 109940134 Chloroplast Carbonylation 4 Rubisco subunit binding-protein β-subunit ORYSA gi 2506277 Chloroplast Carbonylation 4-4 -
Rubisco-activase splice form 1 ARATH gi 12643259 Chloroplast Carbonylation 6 Rubisco-activase splice form 2 ARATH gi 7438144 Chloroplast Carbonylation 6 Rubisco-large subunit ARATH gi 7525041 Chloroplast Carbonylation 6 S-Adenosylmethionine synthetase, putative ARATH AAO11581 Nitrosylation 1 Serine hydroxymethyltransferase (2.1.2.1)* ORYSA gi 1707998 Mit. Matrix Carbonylation 4 SET domain transcriptional regulatory family ARATH Q8VY03 Nitrosylation 1 Storage proteins 7S ARATH At3g22640 Secreted* Carbonylation 5 Succinyl CoA ligase alpha subunit ARATH At5g08300 Chloroplast* Fragment 3 Succinyl CoA ligase beta subunit ARATH At2g20420 Mit.* Fragment 3 Succinyl-CoA-ligase, α-subunit (6.2.1.5)* ORYSA gi 15241592 Mit. Matrix Carbonylation 4 Succinyl-CoA-ligase, β-subunit (6.2.1.5)* ORYSA gi 15225353 Mit. Matrix Carbonylation 4 Superoxide dismutase (Mn) (EC 1.15.1.1) ORYSA gi 75221505 Mit. Matrix Carbonylation 4 Thioredoxin f1 ARATH AAD35003 Nitrosylation 1 Thiosulfate sulfurtransferase* ORYSA gi 4406372 Mit. Matrix Carbonylation 4 Thylakoid lumenal 16.5 kda protein, chloroplast precursor ARATH O22773 Nitrosylation 1 Transcription activator ARATH At3g52910 Cytosol/nuclear Carbonylation 7 Transitional endoplasmic reticulum ATPase ARATH T48355 Nitrosylation 1 Transketolase ARATH At3g60750 Chloroplast* Carbonylation 5 Transketolase-like protein ARATH gi 75264494 Nitrosylation 1 Translation elongation factor eef-1alpha chain ARATH gi 75319442 Nitrosylation 1 Translational elongation factor TuM ORYSA gi 18001149 Mit. Matrix Carbonylation 4 Translationally controlled tumor protein-like protein ARATH Q8L973 Nitrosylation 1 Trigger factor-related ARATH NP_200333 Nitrosylation 1 Triosephosphate isomerase ARATH gi 13432260 Nitrosylation 1 Tubulin alpha 6 chain ARATH P29511 Nitrosylation 1 Tubulin beta 4 chain ARATH P24636 Nitrosylation 1 Type 2 peroxiredoxin-related ARATH NP_176773 Nitrosylation 1 UDP-Glc pyrophosphorylase ARATH At5g17310 Secreted* Carbonylation 5 Unknown protein ARATH AAK59490 Nitrosylation 1 Unnamed protein product* ORYSA gi 9294558 Chloroplast Carbonylation 4 Unnamed protein product (superoxide dismutase) SOLTU gi 19693 Mit. Trp-oxidation 2 Voltage-dependent anion channel ORYSA gi 18076158 Mit. OM M Carbonylation 4 Voltage-dependent anion channel (porin) ARATH At5g15090 Other* Fragment 3 Zinc-finger, C3HC4 (RING finger) ARATH At5g60710 Nuclear Carbonylation 7 a) Protein names are given as in original references. Minor rearrangements of names have been done in order to obtain proteins in alphabetic order. Proteins marked with an asterisk were only identified as oxidized after in vitro oxidation. b) Indicating the organisms used in respective original study: ARATH, Arabidopsis thaliana; ORYSA, Oryza sativa; PEA, Pisum sativum; RICCO, Ricinus communis; SOLTU, Solanum tuberosum. c) When accession no. or other identifiers was included in the original publication, these are included in the table. In cases where more than one identifier was given, the first one has been included. In cases where the original entry has been discontinued the new entries are indicated. d) Localization of the protein as specified in the original publication, or * based on TargetP. Apopl., apoplast; Cyt., cytosol; ER, endoplasmatic reticulum; IMS, intermembrane space; OMM, outer mitochondrial membrane; Mic., microbodies; Mit., mitochondria; Vac., vacuole. e) Type of protein modification identified. f) Reference: 1. Lindermayr C, Saalbach G, Durner J. 2005. Proteomic identification of S-nitrosylated proteins in Arabidopsis. Plant Physiol. 137: 921-930. 2. Møller IM, Kristensen BK. 2006. Protein oxidation in plant mitochondria detected as oxidized tryptophan. Free Radicals Biol. Med. 40: 430-435. 3. Sweetlove LJ, Heazlewood JL, Herald V, Holtzapffel R, Day DA, Leaver CJ, Millar AH. 2002. The impact of oxidative stress on Arabidopsis mitochondria. Plant Journal 32: 891 904. 4. Kristensen BK, Askerlund P, Bykova NV, Egsgaard H, Møller IM. 2004. Identification of oxidised proteins in the matrix of rice leaf mitochondria by immunoprecipitation and two-dimensional liquid chromatography-tandem mass spectrometry. Phytochemistry 65: 1839-1851. 5. Job C, Rajjou L, Lovigny, Y, Belghazi M, Job D. 2005. Patterns of protein oxidation in Arabidopsis seeds and during germination. Plant Physiol. 138: 790-802. - 5 -
6. Johansson E, Olsson O, Nyström T. 2004. Progression and specificity of protein oxidation in the life cycle of Arabidopsis thaliana. J. Biol. Chem. 279: 22204-22208. 7. Davletova S, Rizhsky L, Liang HJ, Shengqiang Z, Oliver DJ et al. 2005. Cytosolic ascorbate peroxidase 1 is a central component of the reactive oxygen gene network of Arabidopsis. Plant Cell 17: 268 281. 8. Nguyen AT, Donaldson RP. 2005. Metal-catalyzed oxidation induces carbonylation of peroxisomal proteins and loss of enzymatic activities. Arch. Biochem. Biophys. 439: 25-31. 9. Romero-Puertas MC, Palma JM, Gómez M, del Rio LA, Sandalio LM. 2002. Cadmium causes the oxidative modification of proteins in pea plants. Plant Cell Environ. 25: 677-686. - 6 -