Heterofile Antistoffer.

November 2014 Pakningsvedlæg, version 2 Heterofile Antistoffer. Det er vigtigt at du returnerer dine resultater, positive som negative. Herved kan andre få oplysning om robustheden af alle de metoder, som er blevet undersøgt. Kittet indeholder tre prøver, Normal, som ikke er tilsat noget 10 mg/l som er tilsat museantistof, SAM (sheep antimouse), i lav koncentration, og 20 mg/l, som er tilsat museantistof i højere koncentration til det samme serum som Normal. Lav dobbeltbestemmelse af den komponent, hvis analysemetode, som ønskes undersøgt for interferens af heterofile antistoffer på alle tre prøver. Udfyld derfor nedenstående skema så detaljeret og udførligt som muligt, og returner det til DEKS, se adresse og mail i footeren. Komponent Fx som i Labterm eller dens NPU-kode Instrument producent og type Reagens(er) Resultat af 1. bestemmelse af Normal + enhed Resultat af 2. bestemmelse af Normal + enhed Resultat af 1. bestemmelse af 10mg/L + enhed Resultat af 2. bestemmelse af 10mg/L + enhed Resultat af 1. bestemmelse af 20mg/L + enhed Resultat af 2. bestemmelse af 20mg/L + enhed Flere kommentarer: Kommentar Revisionshistorie 2013-09: Første version kun med gamle resultater fra den nordiske undersøgelse. 2014-11: Anden version. Med ny forside til indberetning af resultater til fortsat udbredelse af undersøgelse for interferens af heterofile antistoffer. DEKS Indgang 8, 1. sal Glostrup Hospital 2600 Glostrup Tlf.: 3863 4400 Fax: 4453 5369 deks@deks.dk www.deks.dk

Interferences from heterophilic antibodies 2009 Explanation to the al report The labels of the al report consists of: Component The name of the component measured. I have tried to use an understandable name, but many of these components are new to me, so I have used a consensus name if possible. I presume this will cause no problem for those of you who have submitted results for these components as your result has been given. Producer Name of the company that have manufactured the instrument. Different versions may have been collected in a larger group. Instrument Instrument name and type. Results Number of results for this method. Results Number of al results for this method. M First, for each laboratory for each method the fraction of the mean of Replicate 1 and Replicate 2 for sample +SAM and the mean of Replicate 1 and Replicate 2 for sample Normal has been calculated. M in the report is the mean of all the al fractions for each method. Min Minimum value of fraction for this method. Max Maximum value of fraction for this method. Your result Your result, if you have submitted one, is shown here. If one or both of your results for Normal or +SAM is not al, e.g. <0.1 or low, this is indicated as e.g. <0.1/<0.1 or low/low. If you have made any comments to your result, these are presented just beneath your result. 16/6-2010 For EQAnord Pål Rustad NKK Page 1 of 14

Numerical report Institution: Fürst Medisinsk Laboratorium,, NO-1051 OSLO Contact: Trude Torsnes, ttorsnes@furst.no Comments: Component Producer Instrument Results Results A-Casein Phadia Immunocap 1 1 0.9 0.9 0.9 ACTH Roche cobas 1 1 4.8 4.8 4.8 Siemens Immulite 5 3 1.0 1.0 1.0 AFP Abbott Architect 3 3 1.0 1.0 1.0 Brahms Kryptor 1 1 1.3 1.3 1.3 Diasorin Liaison 1 1 0.9 0.9 0.9 Perkin Elmer AutoDelfia 1 1 1.0 1.0 1.0 Roche cobas 5 5 1.3 1.2 1.4 Modular E 7 7 1.3 1.1 1.7 Siemens Centaur 4 4 0.9 0.9 1.0 Immulite 1 1 1.1 1.1 1.1 A-glatt muskulatur BMD CARIS 1 0 A-lactoglobulin IgA Phadia Immunocap 1 1 1.0 1.0 1.0 A-lactaalbumin IgA Immunocap 1 1 1.0 1.0 1.0 Alcalic Phosphatase Bone (BAP) Metra Unknown: Kitname: Metra BAP kit A-LKM BMD CARIS 1 0 A-mitokondrier CARIS 1 0 1 1 0.9 0.9 0.9 ANA Screen Phadia Tecan Genesis RMP202 1 1 1.0 1.0 1.0 1.0 Anti Jo 1 BioRad BioPlex 1 0 Anti-Cardiolipin IgG Phadia Immunocap 1 1 1.3 1.3 1.3 Anti-Cardiolipin IgM Immunocap 1 1 0.9 0.9 0.9 anti-chrom BioRad BioPlex 1 0 Anti-HBc-Tot Abbott Architect 1 1 1.0 1.0 1.0 Bio Merieux Vidas 1 1 1.0 1.0 1.0 1.1 Anti-HBe Abbott Architect 1 1 1.0 1.0 1.0 Anti-HCV Architect 3 3 0.9 0.9 0.9 Anti-parietalceller BMD CARIS 1 0 anti-pr3 capture Tecan ELISA reader Magellan 1 1 3.2 3.2 3.2 anti-ribo P BioRad BioPlex 1 1 1.0 1.0 1.0 anti-rnp 68 BioPlex 1 0 anti-rnp A BioPlex 1 0 anti-ssa-52 BioPlex 1 0 anti-ssa-60 BioPlex 1 0 anti-ssb BioPlex 1 0 Anti-Tg Siemens Immulite 3 3 0.9 0.9 1.0 Anti-TPO Abbott Architect 1 1 0.5 0.5 0.5 Berthold Technol. LUMAT LB9507 1 1 0.2 0.2 0.2 Brahms Kryptor 3 3 1.8 0.9 3.5 Page 2 of 14

Anti-TPO Roche cobas 1 1 1.0 1.0 1.0 Modular E 1 1 1.0 1.0 1.0 Siemens Centaur 5 5 1.2 1.0 1.5 1.0 Immulite 4 4 1.0 0.9 1.1 BNP Abbott Architect 3 2 1.5 1.5 1.5 Borrelia IgGAb gp41 Borrelia IgMAb gp41 Beckman Coulter Access2 2 2 1.0 1.0 1.0 Unicel 2 2 0.9 0.8 1.0 Siemens Centaur 5 4 1.2 1.1 1.4 DAKO Cytomation Unknown: Kitname: IDEIA Lyme Neuro-borreliosis Unknown: Kitname: IDEIA Lyme Neuro-borreliosis 1 1 0.8 0.8 0.8 1 1 0.9 0.9 0.9 CA 125 Abbott Architect 13 13 1.4 1.3 1.5 Beckman Coulter Unicel 2 2 1.0 1.0 1.0 Brahms Kryptor 1 1 0.8 0.8 0.8 Diasorin Liaison 1 1 1.2 1.2 1.2 In house AutoDelfia 1 1 1.8 1.8 1.8 Roche cobas 11 11 1.1 0.9 1.1 Modular E 9 9 1.1 1.0 1.3 Siemens Centaur 10 10 1.0 0.9 1.1 CA 153 Abbott Architect 6 6 1.0 1.0 1.1 Beckman Coulter Unicel 1 1 1.0 1.0 1.0 Diasorin Liaison 1 1 0.9 0.9 0.9 Roche cobas 3 3 1.0 1.0 1.1 Modular E 5 5 1.0 0.9 1.0 Siemens Centaur 2 2 1.0 1.0 1.0 CA 199 Abbott Architect 4 4 3.1 2.4 4.4 Unicel 1 1 1.0 1.0 1.0 Brahms CA 19-9 100 1 1 1.6 1.6 1.6 Diasorin Liaison 1 1 2.2 2.2 2.2 Roche cobas 3 3 1.1 1.1 1.1 Modular E 3 3 1.1 1.1 1.2 Siemens Centaur 2 2 1.1 1.0 1.2 Immulite 1 1 0.9 0.9 0.9 CA 72-4 Roche Modular E 2 2 1.4 1.3 1.4 Calcitonin Siemens CAMA Carbamazepin Olympus Olympus 680 1 1 1.0 1.0 1.0 1.0 Siemens Centaur 1 1 1.1 1.1 1.1 Cardiolopin IgG Phadia Immunocap 2 2 0.9 0.9 0.9 CCP-ab Immuno 205 1 1 1.0 1.0 1.0 Page 3 of 14

CCP-ab Phadia Immunocap 3 3 1.1 0.9 1.2 0.9 Unknown: Kitname: 1 1 3.2 3.2 3.2 CDT % Siemens BN 1 1 1.0 1.0 1.0 Nefelometri 1 1 1.0 1.0 1.0 CEA Abbott Architect 15 15 1.1 1.0 1.2 Unicel 3 3 1.0 0.9 1.0 Diasorin Liaison 1 1 2.5 2.5 2.5 In house AutoDelfia 1 1 2.7 2.7 2.7 Roche cobas 10 10 1.1 1.0 1.1 Modular E 9 9 1.1 1.0 1.1 Siemens Centaur 10 10 1.0 0.7 1.0 Immulite 1 1 1.1 1.1 1.1 Centromer-ab BioRad BioPlex 1 0 Phadia Immunocap 1 1 1.3 1.3 1.3 Choriongonadotrophin Siemens Centaur 1 1 1.1 1.1 1.1 CK-MB Abbott Architect 6 6 1.7 1.6 1.8 Unicel 1 1 1.0 1.0 1.0 Ortho Vitros 1 1 23.9 23.9 23.9 Roche cobas 22 22 2.8 2.3 3.7 Elecsys 5 5 2.9 1.5 3.7 Modular E 8 8 2.9 2.5 3.3 Siemens Centaur 5 5 1.3 1.2 1.4 1.3 Immulite 1 1 1.1 1.1 1.1 CMVIgG Abbott Architect 1 0 CMVIgM Architect 1 1 1.0 1.0 1.0 COBA Siemens Immulite 1 1 0.9 0.9 0.9 Cortisol Abbott Architect 5 5 1.0 1.0 1.0 Beckman Coulter Unicel 1 1 1.0 1.0 1.0 Roche cobas 5 5 1.0 0.9 1.0 Modular E 4 4 1.0 1.0 1.0 Siemens Centaur 2 2 1.0 0.9 1.0 1.0 C-peptid DiaSorin Liaison 2 2 1.0 1.0 1.0 Perkin Elmer AutoDelfia 2 2 1.0 1.0 1.0 Roche cobas 2 2 1.0 1.0 1.0 Modular E 6 6 0.9 0.8 1.0 Siemens Centaur 5 5 0.9 0.6 1.0 Immulite 4 4 1.0 0.9 1.0 cpsa Centaur 3 3 1.0 1.0 1.0 Crosslaps Roche Modular E 1 1 7.0 7.0 7.0 CRP Ortho Vitros 1 1 1.1 1.1 1.1 Page 4 of 14

CRP Roche cobas 2 2 1.0 1.0 1.0 cobas integra 3 3 1.0 1.0 1.0 Siemens Advia 1650 1 1 1.0 1.0 1.0 Cyfra 21-1 Roche Modular E 1 1 1.5 1.5 1.5 D-dimer Stago Star evolution 2 2 1.0 1.0 1.0 DHEAS Roche cobas 1 1 1.0 1.0 1.0 Modular E 1 1 0.9 0.9 0.9 Siemens RIA 1 1 1.0 1.0 1.0 Digitoxin Roche Modular E 1 0 Siemens Centaur 3 3 0.9 0.6 1.0 Digoxin Roche cobas 7 3 1.0 1.0 1.1 Modular E 1 0 Siemens Centaur 2 2 0.8 0.8 0.9 dsdna BioRad BioPlex 1 1 1.0 1.0 1.0 Phadia autocab 1 1 1.3 1.3 1.3 Immunocap 3 3 0.8 0.8 0.9 0.8 e1 (Cat epitelum) Immunocap 1 1 1.1 1.1 1.1 1.1 E146F2-K57 In house interference assay Victor 1 1 341.5 341.5 341.5 EBNA Diasorin Liaison 1 1 1.0 1.0 1.0 Biotest Unknown: Kitname: Biotest AntiEBV EBNA ELISA 1 0 EBV VCA IgG Diasorin Liaison 1 1 10.1 10.1 10.1 Biotest Unknown: Kitname: Biotest AntiEBV VCA IgG ELISA 1 0 EBV VCA IgM Diasorin Liaison 1 1 1.0 1.0 1.0 Biotest Unknown: Kitname: Biotest AntiEBV VCA IgM ELISA 1 1 0.8 0.8 0.8 ECP Phadia Immunocap 2 2 0.9 0.9 1.0 EliA Celiac IgA Immunocap 1 1 1.0 1.0 1.0 1.0 EliA Celiac IgG Immunocap 1 1 1.3 1.3 1.3 1.3 EliA CENP Immunocap 1 1 1.8 1.8 1.8 1.8 EliA Jo-1 Immunocap 1 1 0.8 0.8 0.8 0.8 EliA La Immunocap 1 1 1.1 1.1 1.1 1.1 EliA RNP Immunocap 1 1 1.0 1.0 1.0 1.0 EliA Ro Immunocap 1 1 1.0 1.0 1.0 1.0 EliA Scl-70 Immunocap 1 1 1.0 1.0 1.0 1.1 Page 5 of 14

EliA Sm Phadia Immunocap 1 1 0.8 0.8 0.8 0.8 Enolase DiaSorin Liaison 1 1 1.0 1.0 1.0 EPO Siemens Immulite 3 3 0.9 0.9 1.0 Ferritin Abbott Architect 16 16 1.0 0.9 1.0 Beckman Coulter Access2 1 1 0.9 0.9 0.9 Unicel 4 4 1.0 0.9 1.0 Ortho Vitros 1 1 1.0 1.0 1.0 Roche cobas 27 27 1.0 1.0 1.1 Elecsys 3 3 1.0 1.0 1.0 Modular E 15 15 1.0 1.0 1.1 Siemens Centaur 18 18 1.0 0.9 1.1 Immulite 3 3 1.0 1.0 1.0 Folate Abbott Architect 10 10 1.0 0.9 1.0 Beckman Coulter Access2 1 1 0.9 0.9 0.9 Unicel 3 3 0.9 0.9 0.9 Roche cobas 9 9 1.0 0.9 1.1 Elecsys 2 2 1.1 1.0 1.1 Modular E 4 4 1.0 0.9 1.0 Siemens Centaur 1 1 1.0 1.0 1.0 Immulite 3 3 1.0 1.0 1.0 Free beta-hcg Brahms Kryptor 2 0 Free PSA Abbott Architect 8 8 1.0 0.9 1.0 Beckman Coulter Access2 1 1 1.4 1.4 1.4 Unicel 2 2 1.5 1.3 1.7 Perkin Elmer AutoDelfia 1 1 1.0 1.0 1.0 Roche cobas 11 11 1.1 1.0 1.2 Modular E 9 9 1.1 0.6 1.3 Siemens Immulite 2 2 1.0 1.0 1.0 Free T3 Abbott Architect 6 6 1.0 1.0 1.0 Beckman Coulter Unicel 1 1 0.9 0.9 0.9 Perkin Elmer AutoDelfia 1 1 1.0 1.0 1.0 Roche cobas 7 7 1.0 1.0 1.1 Elecsys 1 1 1.0 1.0 1.0 Modular E 3 3 1.0 1.0 1.0 Siemens Centaur 12 12 0.5 0.2 2.7 0.3 Immulite 2 2 1.1 0.8 1.4 Free T4 Abbott Architect 17 17 1.0 0.9 1.1 Beckman Coulter Access2 1 1 1.1 1.1 1.1 Unicel 5 5 0.9 0.9 1.0 Perkin Elmer AutoDelfia 1 1 1.0 1.0 1.0 Roche cobas 11 11 1.0 1.0 1.0 Elecsys 3 3 1.0 1.0 1.0 Modular E 6 6 1.0 1.0 1.0 Siemens Centaur 9 8 1.0 0.9 1.0 Page 6 of 14

Free T4 Siemens Immulite 4 4 1.0 0.9 1.0 FSH Abbott Architect 6 6 1.0 0.9 1.0 Beckman Coulter Access2 1 1 0.9 0.9 0.9 Unicel 4 4 1.0 0.9 1.0 Perkin Elmer AutoDelfia 3 3 1.0 1.0 1.0 Roche cobas 9 9 0.3 0.3 0.4 Elecsys 1 1 0.3 0.3 0.3 Modular E 10 10 0.3 0.3 0.4 Siemens Centaur 11 11 1.0 0.9 1.0 1.0 Immulite 2 2 1.0 0.9 1.0 Ftin Roche cobas 1 1 1.0 1.0 1.0 Gastrin Siemens Immulite 1 1 1.1 1.1 1.1 GBM-ab BioRad BioPlex 1 0 Phadia Immunocap 2 2 0.9 0.7 1.1 Gentamycin Siemens Centaur 1 1 1.0 1.0 1.0 GH Beckman Coulter Unicel 1 1 1.0 1.0 1.0 DiaSorin Liaison 1 1 1.1 1.1 1.1 Perkin Elmer AutoDelfia 3 3 4.0 3.4 5.0 Siemens Immulite 7 7 1.0 0.8 1.0 Gliadin IgA Phadia Immunocap 2 2 1.0 0.9 1.0 Granulocyte macrophagecolony stimulating factor BioRad BioPlex 1 1 1.7 1.7 1.7 H. p. IgG Siemens HAVAb Centaur 1 1 1.0 1.0 1.0 HAVAb IgG Abbott Architect 4 4 1.0 1.0 1.1 1.1 S/CO= Sample RLU/Cut-off RLU HAVAb IgM Architect 4 4 1.0 0.9 1.1 1.1 Siemens Centaur 1 1 3.6 3.6 3.6 HBcAb-IgM Abbott Architect 1 1 1.0 1.0 1.0 S/CO= Sample RLU/Cut-off RLU HBeAg Architect 4 4 3.6 2.6 4.3 4.3 S/CO= Sample RLU/Cut-off RLU HBsAg Architect 8 8 3.8 2.7 5.0 3.2 Siemens Centaur 1 1 1.5 1.5 1.5 HBVeAb Abbott Architect 1 1 1.0 1.0 1.0 hcg Architect 9 7 1.7 1.5 1.9 Unicel 3 3 1.0 1.0 1.0 BRAHMs Kryptor 1 0 Ortho Vitros 1 1 23.2 23.2 23.2 Roche cobas 28 26 1.3 0.5 2.3 Elecsys 4 4 1.8 1.1 2.7 Modular E 12 10 1.5 1.1 2.0 Siemens Centaur 8 7 1.0 0.9 1.3 1.1 Page 7 of 14

hcg Siemens Immulite 6 6 1.0 1.0 1.0 hcg beta fritt BRAHMS Kryptor 1 0 Perkin Elmer AutoDelfia 1 1 5.6 5.6 5.6 HCVAb Siemens Centaur 1 1 0.9 0.9 0.9 Helicobacter Pylori IgG Bio Merieux Vidas 1 1 0.9 0.9 0.9 0.9 Her-2 Siemens Centaur 1 1 1.1 1.1 1.1 Heterophilic ab Orion Unknown: Kitname: Clearview IM 1 0 HIV Abbott Architect 1 1 2.2 2.2 2.2 OBS! Positive sample H.Pylori HIV Ag/Ab Architect 5 5 1.4 1.1 2.1 2.1 Homocystein Architect 2 2 1.0 1.0 1.0 S/CO= Sample RLU/Cut-off RLU Siemens Centaur 4 4 1.0 1.0 1.1 1.0 Immulite 2 2 1.0 0.9 1.1 IGE Beckman Coulter Unicel 1 1 0.9 0.9 0.9 Phadia autocab 1 1 1.0 1.0 1.0 Immunocap 2 2 1.0 0.9 1.0 Roche cobas 2 2 1.0 1.0 1.0 Modular E 4 4 1.0 1.0 1.0 Siemens Centaur 2 2 1.0 1.0 1.0 Immulite 2 2 1.0 0.9 1.0 IgE mod Birk Phadia autocab 1 1 0.9 0.9 0.9 IGF-1 Siemens Immulite 5 5 0.9 0.8 1.0 IGFBP1 Medix Biochemica Unknown: Kitname: IEMA Test 1 1 0.9 0.9 0.9 IGFBP-3 Siemens Immulite 3 3 1.0 1.0 1.0 Inhibin A Beckman Coulter Unicel 1 1 1.2 1.2 1.2 Insulin Unicel 1 1 1.0 1.0 1.0 Perkin Elmer AutoDelfia 2 2 1.0 1.0 1.0 Roche cobas 2 2 1.0 1.0 1.0 Modular E 5 5 1.0 1.0 1.1 Siemens Centaur 2 2 1.0 0.9 1.0 Immulite 2 2 1.5 1.0 2.0 Interferon gamma BioRad BioPlex 1 1 3.9 3.9 3.9 Interleukin 10 BioPlex 1 1 2.6 2.6 2.6 Siemens Immulite 1 1 12.1 12.1 12.1 Interleukin 12 BioRad BioPlex 1 1 3.7 3.7 3.7 Interleukin 13 BioPlex 1 1 0.9 0.9 0.9 Interleukin 1beta BioPlex 1 1 3.6 3.6 3.6 Siemens Immulite 2 2 1.1 1.0 1.2 Interleukin 2 BioRad BioPlex 1 1 7.3 7.3 7.3 Interleukin 4 BioPlex 1 1 0.9 0.9 0.9 Interleukin 5 BioPlex 1 1 1.3 1.3 1.3 Interleukin 6 BioPlex 1 1 0.8 0.8 0.8 Biosource Immunoassay kit 1 1 0.7 0.7 0.7 Page 8 of 14

Interleukin 6 Roche cobas 3 3 1.3 1.1 1.4 Siemens Immulite 3 2 1.0 1.0 1.0 Interleukin 7 BioRad BioPlex 1 1 1.3 1.3 1.3 Interleukin 8 BioPlex 1 1 0.8 0.8 0.8 K57F2-K57 Siemens Immulite 2 2 1.0 1.0 1.0 In house interference assay Victor 1 1 403.1 403.1 403.1 K57G-K57 Victor 1 1 494.6 494.6 494.6 La-ab (SSB) Phadia Immunocap 1 1 1.0 1.0 1.0 LH Abbott Architect 4 4 1.0 0.9 1.0 Unicel 3 3 0.9 0.9 0.9 Perkin Elmer AutoDelfia 3 3 1.0 1.0 1.0 Roche cobas 10 10 1.0 1.0 1.0 Elecsys 1 1 1.0 1.0 1.0 Modular E 10 10 1.0 1.0 1.0 Siemens Centaur 11 11 0.9 0.8 1.0 0.9 Immulite 3 3 1.0 0.9 1.0 Litium Olympus Olympus 680 1 1 0.9 0.9 0.9 1.0 Lp(a) Siemens BN 1 1 1.0 1.0 1.0 Mb Roche cobas 1 1 1.5 1.5 1.5 MBL In-house Unknown: Kitname: 1 1 1.1 1.1 1.1 MPO-ab BioRad BioPlex 1 0 Phadia Immunocap 2 2 0.9 0.8 0.9 Eurodiagnostica Tecan Genesis RMP200 1 1 0.9 0.9 0.9 0.9 Unknown: Kitname: Wieslab Capture MPO- ANCA IU-kit 1 1 2.3 2.3 2.3 MUC1 In house AutoDelfia 1 1 1.0 1.0 1.0 Myoglobin Abbott Architect 2 2 1.0 1.0 1.0 Beckman Coulter Unicel 1 1 1.0 1.0 1.0 Roche cobas 12 12 1.5 1.4 1.6 Elecsys 3 3 1.8 1.6 1.8 Modular E 4 4 1.5 1.4 1.6 Siemens BN 1 1 1.0 1.0 1.0 Centaur 3 3 1.0 0.9 1.0 Immulite 1 1 0.9 0.9 0.9 NSE Diasorin Liaison 1 1 1.0 1.0 1.0 In house Gammacounter 1 1 1.1 1.1 1.1 Roche cobas 1 1 1.2 1.2 1.2 Elecsys 1 1 1.1 1.1 1.1 Modular E 1 1 1.0 1.0 1.0 NT-ProBNP cobas 16 16 1.0 1.0 1.0 Page 9 of 14

NT-ProBNP Roche Elecsys 2 2 1.0 1.0 1.0 Modular E 10 10 1.0 1.0 1.1 Oestradiol Abbott Architect 3 3 1.0 1.0 1.0 Beckman Coulter Unicel 1 1 1.0 1.0 1.0 Roche cobas 3 3 1.0 1.0 1.1 Modular E 3 3 1.0 1.0 1.0 Siemens Centaur 7 7 1.0 1.0 1.0 1.0 Ostase Beckman Coulter Unicel 1 1 0.5 0.5 0.5 Osteocalsin Roche Elecsys 1 1 1.1 1.1 1.1 Siemens PAPP-A Brahms Kryptor 4 4 4.8 0.1 7.8 Perkin Elmer AutoDelfia 1 1 17.5 17.5 17.5 Parathyrin Roche cobas 1 1 4.9 4.9 4.9 PCT BRAHMS Kryptor 1 1 2.2 2.2 2.2 Roche cobas 2 2 3.1 1.9 4.2 Modular E 1 1 9.1 9.1 9.1 PEP Siemens PGE Centaur 1 1 1.0 1.0 1.0 Phenobarbital Olympus Olympus 680 1 1 1.0 1.0 1.0 1.0 Siemens Centaur 1 1 0.9 0.9 0.9 Phenytoin Olympus Olympus 680 1 1 1.0 1.0 1.0 1.0 Roche cobas 1 1 1.0 1.0 1.0 Modular P 1 1 0.9 0.9 0.9 PLA Siemens Centaur 1 1 1.0 1.0 1.0 PR3-ab BioRad BioPlex 1 0 Phadia Immunocap 2 2 0.8 0.8 0.9 Eurodiagnostica Tecan Genesis RMP201 1 1 1.0 1.0 1.0 1.0 Unknown: Kitname: Wieslab Capture PR3-ANCA IU kit 1 1 2.5 2.5 2.5 ProCalcitonin Roche cobas 4 4 4.5 1.7 6.7 Modular E 1 1 1.7 1.7 1.7 Progesterone Abbott Architect 1 1 1.0 1.0 1.0 Roche cobas 9 9 1.0 0.9 1.3 Elecsys 1 1 1.3 1.3 1.3 Modular E 7 7 1.1 1.0 1.3 Siemens Centaur 4 4 1.0 1.0 1.0 1.0 progrp In house AutoDelfia 1 1 1.7 1.7 1.7 Prolactin Abbott Architect 7 7 1.1 1.0 1.1 Beckman Coulter Access2 1 1 0.8 0.8 0.8 Page 10 of 14

Prolactin Beckman Coulter Unicel 4 4 0.8 0.8 0.8 BRAHMS Kryptor 1 1 0.9 0.9 0.9 Perkin Elmer AutoDelfia 1 1 1.1 1.1 1.1 Roche cobas 10 10 1.0 1.0 1.1 Elecsys 1 1 1.0 1.0 1.0 Modular E 7 7 1.0 1.0 1.1 Siemens Centaur 13 13 1.0 0.9 1.0 0.9 Immulite 2 2 1.0 1.0 1.0 Protein-S Stago Star evolution 1 1 0.9 0.9 0.9 PSA Abbott Architect 20 20 1.0 1.0 1.1 Beckman Coulter Access2 1 1 2.9 2.9 2.9 Unicel 3 3 2.2 2.1 2.3 Ortho Vitros 1 1 1.0 1.0 1.0 Perkin Elmer AutoDelfia 2 2 1.1 1.0 1.1 Roche cobas 27 27 1.0 1.0 1.1 Elecsys 3 3 1.0 1.0 1.0 Modular E 16 16 1.0 1.0 1.3 Siemens Centaur 13 13 1.0 0.9 1.0 1.0 Immulite 5 5 1.0 1.0 1.0 PTH Abbott Architect 2 2 1.0 1.0 1.1 Unicel 1 1 1.0 1.0 1.0 Roche cobas 16 16 5.7 0.2 7.3 Elecsys 1 1 7.8 7.8 7.8 Modular E 11 11 7.1 5.1 11.0 Siemens Centaur 3 2 1.0 1.0 1.0 Immulite 5 5 1.0 0.8 1.1 Renin DiaSorin Liaison 1 1 0.9 0.9 0.9 CIS bio RIA 1 1 1.4 1.4 1.4 Ro-ab (SSA) Phadia Immunocap 1 1 1.3 1.3 1.3 Rubella Abbott Architect 2 2 1.0 1.0 1.1 Diasorin Liaison 1 1 1.0 1.0 1.0 Rubella IgM Liaison 1 1 1.1 1.1 1.1 S-100 Roche cobas 2 2 1.3 1.2 1.4 Elecsys 1 1 1.3 1.3 1.3 Modular E 1 1 1.3 1.3 1.3 Scl-70 BioRad BioPlex 1 0 Phadia Immunocap 1 1 1.0 1.0 1.0 SHBG Abbott Architect 1 1 1.0 1.0 1.0 Beckman Coulter Access2 1 1 1.1 1.1 1.1 Unicel 1 1 1.0 1.0 1.0 Perkin Elmer Wizard 1470 1 1 1.1 1.1 1.1 Roche cobas 7 7 1.0 1.0 1.1 Page 11 of 14

SHBG Roche Modular E 5 5 1.0 1.0 1.0 Siemens Immulite 8 7 1.0 0.9 1.1 1.1 sil-2r Immulite 1 1 1.9 1.9 1.9 Sm-ab BioRad BioPlex 1 0 Phadia Immunocap 1 1 1.0 1.0 1.0 stfr Roche cobas integra 1 1 1.0 1.0 1.0 Siemens BN 1 1 1.0 1.0 1.0 Symphoni Phadia autocab 1 1 1.2 1.2 1.2 Immunocap 1 1 1.3 1.3 1.3 Syphilis Abbott Architect 1 1 1.0 1.0 1.0 T3 Architect 2 2 1.0 1.0 1.0 Beckman Coulter Unicel 1 1 0.9 0.9 0.9 Perkin Elmer AutoDelfia 1 1 0.3 0.3 0.3 Roche cobas 4 4 1.0 1.0 1.0 Modular E 2 2 1.0 1.0 1.0 Siemens Centaur 3 3 1.0 1.0 1.1 Immulite 2 2 1.0 0.9 1.0 T4 Abbott Architect 2 2 1.0 1.0 1.0 T84F2-K57 Beckman Coulter Unicel 1 1 0.7 0.7 0.7 Perkin Elmer AutoDelfia 1 1 0.7 0.7 0.7 Roche cobas 2 2 1.0 1.0 1.0 Modular E 2 2 1.0 1.0 1.0 Siemens Centaur 3 3 1.0 1.0 1.0 In house interference assay Victor 1 1 433.1 433.1 433.1 T84F-K57 Victor 1 1 213.5 213.5 213.5 T84G-K57 Victor 1 1 737.5 737.5 737.5 T84hF2-K57 Victor 1 1 9.9 9.9 9.9 T84hF-K57 Victor 1 1 9.6 9.6 9.6 T84scFv-K57 Victor 1 1 8.2 8.2 8.2 TATI (tumour-associated trypsin inhibitor) Perkin Elmer Wizard 1470 1 1 0.8 0.8 0.8 TBG Siemens TBhCG2 Unicel 1 1 1.0 1.0 1.0 Testosterone Abbott Architect 1 1 1.0 1.0 1.0 Beckman Coulter Access2 1 1 0.9 0.9 0.9 Unicel 2 2 0.9 0.9 1.0 Roche cobas 7 7 1.0 0.9 1.1 Elecsys 1 1 1.1 1.1 1.1 Modular E 4 4 1.0 1.0 1.0 Siemens Centaur 3 3 1.0 1.0 1.1 1.0 TGAB Brahms Kryptor 2 2 0.8 0.6 1.0 Page 12 of 14

ThCG Siemens Centaur 3 3 1.0 1.0 1.1 Thyroglobulin BRAHMS Kryptor 1 1 1.1 1.1 1.1 In house AutoDelfia 1 1 3.6 3.6 3.6 Roche Modular E 1 1 1.1 1.1 1.1 Siemens Immulite 2 2 1.0 0.9 1.0 TNF alpha Immulite 2 2 1.0 1.0 1.1 Tobramycin Centaur 1 1 1.0 1.0 1.0 Toxo IgG Diasorin Liaison 1 1 1.0 1.0 1.0 Toxo IgM Liaison 1 1 1.1 1.1 1.1 Roche cobas 1 1 1.0 1.0 1.0 Transaminase IgA Phadia Immunocap 1 1 1.0 1.0 1.0 Transaminase IgG Immunocap 1 1 0.8 0.8 0.8 TRANSGLU Immunocap 2 2 1.0 0.9 1.0 TRAS Roche Modular E 2 2 3.8 0.8 6.8 RSR Limited Unknown: Kitname: Trab ELISA 3A 1 1 1.0 1.0 1.0 Triiodothyronine-reaction Siemens Centaur 1 1 1.0 1.0 1.0 Troponin I Abbott Architect 15 13 1.0 0.3 1.6 Beckman Coulter Access2 2 2 1.5 1.0 2.0 Unicel 4 4 1.2 1.0 1.5 Ortho Vitros 1 1 4.8 4.8 4.8 Radiometer AQT90 Flex 1 0 Siemens Centaur 9 7 20.3 0.9 135.0 Stratus CS 2 2 21.1 15.3 27.0 Troponin T Roche cobas 26 21 2.4 0.3 6.2 Elecsys 5 2 1.4 1.3 1.6 h232 1 0 Modular E 7 6 2.5 1.1 4.7 Troponin T-hs cobas 10 10 2.5 1.2 6.6 Elecsys 1 1 4.2 4.2 4.2 Modular E 4 4 3.8 1.7 4.6 Tryptase Phadia Immunocap 2 2 1.3 0.5 2.0 TSH Abbott Architect 26 26 1.3 0.8 1.3 Unicel 6 6 1.0 0.9 1.0 Ortho Vitros 1 1 4.2 4.2 4.2 Perkin Elmer AutoDelfia 3 3 2.1 1.9 2.3 Roche cobas 33 33 1.0 1.0 1.1 Elecsys 4 4 1.0 1.0 1.0 Modular E 20 20 1.0 1.0 1.1 Siemens Centaur 21 21 0.9 0.6 1.0 1.0 Immulite 4 4 0.9 0.9 1.0 Tumor necrosis factor alpha BioRad BioPlex 1 1 1.0 1.0 1.0 T-Uptake Roche cobas 2 2 1.0 1.0 1.0 Page 13 of 14

T-Uptake Siemens Centaur 1 1 1.0 1.0 1.0 U1RNP-ab Phadia Immunocap 1 1 0.9 0.9 0.9 Valproic Acid Abbott Architect 1 1 1.0 1.0 1.0 Olympus Olympus 680 1 1 1.0 1.0 1.0 1.0 Roche Modular P 1 1 0.9 0.9 0.9 Siemens Immulite 2 1 1.0 1.0 1.0 Vancomycin Abbott Architect 1 1 1.0 1.0 1.0 Siemens Centaur 1 1 1.0 1.0 1.0 Vitamin B12 Abbott Architect 4 4 1.0 1.0 1.1 Beckman Coulter Access2 2 2 1.0 1.0 1.1 Unicel 3 3 1.0 1.0 1.0 Roche cobas 8 8 1.0 1.0 1.0 Elecsys 2 2 1.1 1.0 1.1 Modular E 4 4 1.0 1.0 1.0 Siemens Centaur 1 1 1.0 1.0 1.0 Vitamin D Diasorin Liaison 1 1 1.0 1.0 1.0 Vttg - IgA Phadia Immuno 205 1 1 1.0 1.0 1.0 VWF:Ag Siemens BCS XP 1 1 1.0 1.0 1.0 Page 14 of 14