DL-methionine supplementation of rice-and-bean diets affects gamma-glutamyltranspeptidase activity and glutathione content in livers of growing rats

Transkript

1 Brazilian GGT activity Journal and of GSH Medical levels and in growing Biological rats Research eating rice-and-bean (1999) 32: diets ISSN X Short Communication 483 DL-methionine supplementation of rice-and-bean diets affects gamma-glutamyltranspeptidase activity and glutathione content in livers of growing rats I.M.V. De-Oliveira, E. Fujimori, V.G. Pereira and V.D. De-Castro Laboratório de Nutrição, Departamento de Enfermagem em Saúde Coletiva, Escola de Enfermagem, Universidade de São Paulo, São Paulo, SP, Brasil Correspondence I.M.V. De-Oliveira Departamento de Enfermagem em Saúde Coletiva Escola de Enfermagem, USP Av. Dr. Enéas C. Aguiar, São Paulo, SP Brasil Fax: Research supported by FAPESP (No. 95/0543-4). Received March 3, 1998 Accepted November 30, 1998 Abstract Key words Liver gamma glutamyltranspeptidase Liver glutathione Protein deficiency Rice-and-bean diet Growing rats DL-methionine supplementation

2 484 I.M.V. De-Oliveira et al. g

3 GGT activity and GSH levels in growing rats eating rice-and-bean diets 485 Table 1 - Effect of dietary protein on biological parameters of rats during growth (22-50 days of age). Data are reported as means ± SD (N = 5-18). In any one column, the values of the means marked with different superscripts are significantly different (P<0.05, ANOVA test for two groups). Food efficiency = weight gain/food intake; DL-met = DL-methionime. Diets Initial body Weight gain Food intake Protein intake Food weight (g) (g) (g/day) (g/day) efficiency 13% Protein Casein ± 3.84 a ± a ± 1.08 a 1.96 ± 0.14 a 0.38 ± 0.05 a Rice-bean ± 3.98 a ± 3.95 b ± 0.72 b 1.33 ± 0.10 b 0.21 ± 0.01 b Rice-bean + DL-met ± 2.90 a ± c ± 1.02 c 1.62 ± 0.13 c 0.33 ± 0.01 c 6% Protein Casein ± 3.72 a ± 6.60 c ± 1.04 a 0.86 ± 0.06 d 0.30 ± 0.01 d Rice-bean ± 3.39 a ± 3.79 d 6.85 ± 0.81 d 0.41 ± 0.05 e 0.10 ± 0.02 e Rice-bean + DL-met ± 4.41 a ± 8.28 e 9.70 ± 1.08 b 0.58 ± 0.07 f 0.17 ± 0.02 f

4 486 I.M.V. De-Oliveira et al. Table 2 - Effect of protein restriction on GGT activity, GSH concentration and protein concentration in the liver of rats during growth (22-50 days of age). Data are reported as means ± SD (N = 5-18). In any one column, the values of the means marked with different superscripts are significantly different (P<0.05, ANOVA test for two groups). U = µmol of p-nitroaniline released/min, at 37 o C; DL-met = DLmethionine. Diets GGT GSH (µmol/g Protein (mg/g (U/g protein) wet weight) wet weight) 13% Protein Casein 0.40 ± 0.25 a 3.66 ± 1.17 a ± ab Rice-bean 1.56 ± 0.67 bd 1.74 ± 0.15 b ± a Rice-bean + DL-met 0.79 ± 0.16 c 5.51 ± 0.27 c ± ab 6% Protein Casein 0.96 ± 0.84 cd 3.48 ± 0.34 a ± ab Rice-bean 2.17 ± 0.74 b 1.13 ± 0.11 d ± ab Rice-bean + DL-met 1.85 ± 1.23 bc 5.19 ± 0.54 c ± b

5 GGT activity and GSH levels in growing rats eating rice-and-bean diets 487

6 488 I.M.V. De-Oliveira et al. Acknowledgments References 1. Anderson ME (1996). Glutathione. In: Punchard NA & Kelly FJ (Editors), Free Radicals: A Practical Approach. IRL Press, Oxford. 2. Comporti M, Maellaro E, Del Bello B & Casini AF (1991). Glutathione depletion: its effect on other antioxidant systems and hepatocellular damage. Xenobiotica, 21: Beatty PW & Reed DJ (1980). Involvement of the cystathionine pathway in the biosynthesis of glutathione by isolated rat hepatocytes. Archives of Biochemistry and Biophysics, 204: Meister A (1991). Glutathione deficiency produced by inhibition of its synthesis, and its reversal applications in research and therapy. Pharmacology and Therapeutics, 51: Stipanuk MH (1992). Cysteine concentration regulates cysteine metabolism to glutathione, sulfate and taurine in rat hepatocytes. Journal of Nutrition, 122: Bauman P, Smith TK & Bray TM (1988). The effect of dietary protein and sulfur amino acids on hepatic glutathione concentration and glutathione dependent enzyme activity in the rat. Canadian Journal of Physiology and Pharmacology, 66: Hum S, Koski KG & Hoffer LJ (1992). Varied protein intake alters glutathione metabolism in rats. Journal of Nutrition, 122: Goss PM, Bray TM & Nagy LE (1994). Regulation of hepatocyte glutathione by amino acid precursors and camp in protein-energy malnourished rats. Journal of Nutrition, 124: Cho ES, Johnson N & Snider BCF (1984). Tissue glutathione as a cyst(e)ine reservoir during cystine depletion in growing rats. Journal of Nutrition, 114: Speisky H, Shackel N, Varghese G, Wade D & Israel Y (1990). Role of hepatic g- glutamyltransferase in the degradation of circulating glutathione: Studies in the intact guinea pig perfused liver. Hepatology, 11: White AC, Thannickal VJ & Fanburg BL (1994). Glutathione deficiency in human disease. Journal of Nutritional Biochemistry, 5: Hanigan M & Pitot H (1985). Gammaglutamyltranspeptidase - its role in hepatocarcinogenesis. Carcinogenesis, 6: Antener I, Givel F, Mauron J & Verwilghen AM (1980). Measurement of serum isoenzymes of g-glutamyl-transpeptidase in malnutrition. Acta Paediatrica, 33: Reeves PG, Nielsen FH & Fahey Jr GG (1993). AIN-93 Purified diets for laboratory rodents: Final report of the American Institute of Nutrition Ad Hoc Writing Committee on the Reformulation of AIN-76A Rodent diet. Journal of Nutrition, 123: Persijn JP (1971). Colorimetric assay for g- glutamyl transpeptidase. Clinica Chimica Acta, 35: Saville B (1958). A scheme for the colorimetric determination of microgram a- mounts of thiols. Analyst, 83: Lowry OH, Rosebrough NJ, Farr AL & Randall RJ (1951). Protein measurement with Folin phenol reagent. Journal of Biological Chemistry, 19: Higashi T, Tateishi N, Naruse A & Sakamoto Y (1977). A novel physiological role of liver glutathione as reservoir of L- cysteine. Journal of Biochemistry, 82: Oliveira IMV & De Angelis RC (1996). Differential effects of protein restriction gamma-glutamyltranspeptidase (EC ) activity in young and mature rats. Brazilian Journal of Medical and Biological Research, 29: Jahoor F (1995). Protein-deficient pigs cannot maintain reduced glutathione homeostasis when subjected to the stress of inflammation. Journal of Nutrition, 125: